Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19279 | 3' | -47.9 | NC_004685.1 | + | 6571 | 0.66 | 0.991564 |
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Target: 5'- uGGCAgcUCGgcaccgGCUGGgCGAGcAGUUCg -3' miRNA: 3'- gUUGUa-AGCaa----CGACCaGCUC-UCAAG- -5' |
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| 19279 | 3' | -47.9 | NC_004685.1 | + | 23897 | 0.67 | 0.983197 |
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Target: 5'- aGACGUUCGUggugaagugGCUGGgCGAGGuGUa- -3' miRNA: 3'- gUUGUAAGCAa--------CGACCaGCUCU-CAag -5' |
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| 19279 | 3' | -47.9 | NC_004685.1 | + | 45243 | 0.67 | 0.983197 |
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Target: 5'- aCGGCGaccaGUUGCgGGUCGGGGGg-- -3' miRNA: 3'- -GUUGUaag-CAACGaCCAGCUCUCaag -5' |
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| 19279 | 3' | -47.9 | NC_004685.1 | + | 11793 | 0.67 | 0.975801 |
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Target: 5'- aGGCg--CGgcGCaUGGUCGAaGAGUUCa -3' miRNA: 3'- gUUGuaaGCaaCG-ACCAGCU-CUCAAG- -5' |
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| 19279 | 3' | -47.9 | NC_004685.1 | + | 69180 | 0.72 | 0.864023 |
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Target: 5'- aGGCAUUCGUUGCcgaaaUGGUCGccuacGGGUUg -3' miRNA: 3'- gUUGUAAGCAACG-----ACCAGCu----CUCAAg -5' |
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| 19279 | 3' | -47.9 | NC_004685.1 | + | 65085 | 1.12 | 0.004711 |
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Target: 5'- aCAACAUUCGUUGCUGGUCGAGAGUUCg -3' miRNA: 3'- -GUUGUAAGCAACGACCAGCUCUCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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