Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19280 | 5' | -60.7 | NC_004685.1 | + | 50507 | 0.66 | 0.490399 |
Target: 5'- cCGGCA-GCCCCGgagccauccaucuccUCCcacaGCCCGaCGCCg -3' miRNA: 3'- uGUUGUaCGGGGU---------------AGG----CGGGC-GCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 48236 | 0.66 | 0.486468 |
Target: 5'- aAC-ACcgGCCgCCAguagCCGCCUGUGCa- -3' miRNA: 3'- -UGuUGuaCGG-GGUa---GGCGGGCGCGga -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 48210 | 0.66 | 0.486468 |
Target: 5'- cGCggUAUGUCUCga-CGCCCGUGUCg -3' miRNA: 3'- -UGuuGUACGGGGuagGCGGGCGCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 58065 | 0.66 | 0.467043 |
Target: 5'- -gGugGUGUCCCA-CCGgCUGCGCUc -3' miRNA: 3'- ugUugUACGGGGUaGGCgGGCGCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 12430 | 0.66 | 0.448027 |
Target: 5'- aGCGGCuGUGaCCgCCcgCCGCCgaGCGCCc -3' miRNA: 3'- -UGUUG-UAC-GG-GGuaGGCGGg-CGCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 56879 | 0.66 | 0.448027 |
Target: 5'- gACGGCAcGCCCgCcagCCGCUCG-GCCUg -3' miRNA: 3'- -UGUUGUaCGGG-Gua-GGCGGGCgCGGA- -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 51665 | 0.66 | 0.448027 |
Target: 5'- uGCGGCAgcGCCUCGUCggUGCCCGCGg-- -3' miRNA: 3'- -UGUUGUa-CGGGGUAG--GCGGGCGCgga -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 29088 | 0.67 | 0.438681 |
Target: 5'- -gAugGUGCCgCCGagggaaCCGgCCGCGCCg -3' miRNA: 3'- ugUugUACGG-GGUa-----GGCgGGCGCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 16127 | 0.67 | 0.438681 |
Target: 5'- cACGGCcacgGuCCCCuggagucggCCGCgCCGCGCCa -3' miRNA: 3'- -UGUUGua--C-GGGGua-------GGCG-GGCGCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 11346 | 0.67 | 0.433127 |
Target: 5'- gGCGACccGCCCCGacaccgaggcugugCCGgCCGgGCCg -3' miRNA: 3'- -UGUUGuaCGGGGUa-------------GGCgGGCgCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 34464 | 0.67 | 0.420329 |
Target: 5'- cGCcGCAcGUCuCCAUCCGCaCCGUGCg- -3' miRNA: 3'- -UGuUGUaCGG-GGUAGGCG-GGCGCGga -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 9583 | 0.67 | 0.420329 |
Target: 5'- aACGACGuguucuccUGCCCCAUCgGUUCGgGCg- -3' miRNA: 3'- -UGUUGU--------ACGGGGUAGgCGGGCgCGga -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 58635 | 0.67 | 0.420329 |
Target: 5'- cCGACAUGCgU--UCgGCCCGCGUCg -3' miRNA: 3'- uGUUGUACGgGguAGgCGGGCGCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 30076 | 0.67 | 0.411329 |
Target: 5'- uCGGCAa-CCUguUCCGCgCCGCGCCg -3' miRNA: 3'- uGUUGUacGGGguAGGCG-GGCGCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 10099 | 0.67 | 0.411329 |
Target: 5'- cGCAGCcgGUGCgcucaCCCGccgCCGCCUGCGCa- -3' miRNA: 3'- -UGUUG--UACG-----GGGUa--GGCGGGCGCGga -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 201 | 0.67 | 0.403332 |
Target: 5'- uCGAcCAUGCCCCGcgucaucaaaggcgaCUGCCCGCGUUUg -3' miRNA: 3'- uGUU-GUACGGGGUa--------------GGCGGGCGCGGA- -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 66795 | 0.68 | 0.376554 |
Target: 5'- gGCcGgGUGCCCgAUCCugGCCaGCGCCg -3' miRNA: 3'- -UGuUgUACGGGgUAGG--CGGgCGCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 2551 | 0.68 | 0.368175 |
Target: 5'- aGguGCGUGCCCUccaggCUGCaCUGCGCCg -3' miRNA: 3'- -UguUGUACGGGGua---GGCG-GGCGCGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 20794 | 0.68 | 0.368175 |
Target: 5'- cGCGGCA-GCCCCGUCgGCaCCGagGUCg -3' miRNA: 3'- -UGUUGUaCGGGGUAGgCG-GGCg-CGGa -5' |
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19280 | 5' | -60.7 | NC_004685.1 | + | 20298 | 0.68 | 0.359925 |
Target: 5'- gACGGCGUGCCCUacGUCUaugucgauguGCCCGacggGCCg -3' miRNA: 3'- -UGUUGUACGGGG--UAGG----------CGGGCg---CGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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