Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19288 | 5' | -51.2 | NC_004685.1 | + | 3904 | 0.66 | 0.936139 |
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Target: 5'- -cAC-CGUCuGGCCUUCG-GGugUGAg -3' miRNA: 3'- caUGaGUAG-CUGGAAGCuCCugACUa -5' |
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| 19288 | 5' | -51.2 | NC_004685.1 | + | 69512 | 0.66 | 0.918758 |
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Target: 5'- --gUUCAUCGACCgacUGAGGuccgACUGAUg -3' miRNA: 3'- cauGAGUAGCUGGaa-GCUCC----UGACUA- -5' |
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| 19288 | 5' | -51.2 | NC_004685.1 | + | 34850 | 0.66 | 0.91239 |
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Target: 5'- -gACUCGuUCGACUacgagUUCGAGGACg--- -3' miRNA: 3'- caUGAGU-AGCUGG-----AAGCUCCUGacua -5' |
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| 19288 | 5' | -51.2 | NC_004685.1 | + | 41404 | 0.67 | 0.898801 |
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Target: 5'- -cGCUCAUCG-CCUacaUCGAcgacgcggacGGACUGGc -3' miRNA: 3'- caUGAGUAGCuGGA---AGCU----------CCUGACUa -5' |
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| 19288 | 5' | -51.2 | NC_004685.1 | + | 28697 | 0.71 | 0.722694 |
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Target: 5'- -gGCUUGcCGACCUUCGAGG-UUGAa -3' miRNA: 3'- caUGAGUaGCUGGAAGCUCCuGACUa -5' |
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| 19288 | 5' | -51.2 | NC_004685.1 | + | 13378 | 0.77 | 0.374027 |
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Target: 5'- -aGCUCGccUCGGCCaUCGAGGACUGu- -3' miRNA: 3'- caUGAGU--AGCUGGaAGCUCCUGACua -5' |
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| 19288 | 5' | -51.2 | NC_004685.1 | + | 59401 | 1.06 | 0.0048 |
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Target: 5'- gGUACUCAUCGACCUUCGAGGACUGAUa -3' miRNA: 3'- -CAUGAGUAGCUGGAAGCUCCUGACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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