Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19289 | 5' | -52.7 | NC_004685.1 | + | 53814 | 0.66 | 0.921129 |
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Target: 5'- uCGUCGgguguguagcccUCCUGAAGGUcGGccg-GUGGCg -3' miRNA: 3'- -GCAGC------------AGGGCUUCUA-CCuugaCACCG- -5' |
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| 19289 | 5' | -52.7 | NC_004685.1 | + | 15999 | 0.66 | 0.895241 |
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Target: 5'- gCGUCGUCCCGccguGGcUGGucaacgucgAGCUGaagaagGGCg -3' miRNA: 3'- -GCAGCAGGGCu---UCuACC---------UUGACa-----CCG- -5' |
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| 19289 | 5' | -52.7 | NC_004685.1 | + | 43009 | 0.66 | 0.887382 |
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Target: 5'- uCGUCaUCCCGAugagcuGAUGcGcgcgcucGGCUGUGGUg -3' miRNA: 3'- -GCAGcAGGGCUu-----CUAC-C-------UUGACACCG- -5' |
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| 19289 | 5' | -52.7 | NC_004685.1 | + | 44971 | 0.67 | 0.865201 |
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Target: 5'- aGUCGUCgUGuc---GGAGCUGUGGg -3' miRNA: 3'- gCAGCAGgGCuucuaCCUUGACACCg -5' |
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| 19289 | 5' | -52.7 | NC_004685.1 | + | 65875 | 0.67 | 0.857081 |
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Target: 5'- cCGUCG-CCCGAcgAGAUGcGAGCcagcacGGCa -3' miRNA: 3'- -GCAGCaGGGCU--UCUAC-CUUGaca---CCG- -5' |
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| 19289 | 5' | -52.7 | NC_004685.1 | + | 13195 | 0.68 | 0.822372 |
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Target: 5'- cCGUCGUCCCGAAcccGAaucGGAGCagcccaagGGCc -3' miRNA: 3'- -GCAGCAGGGCUU---CUa--CCUUGaca-----CCG- -5' |
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| 19289 | 5' | -52.7 | NC_004685.1 | + | 67856 | 0.68 | 0.822372 |
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Target: 5'- --cCGUCCCGAGGucgacAUGGGAUcGcGGCg -3' miRNA: 3'- gcaGCAGGGCUUC-----UACCUUGaCaCCG- -5' |
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| 19289 | 5' | -52.7 | NC_004685.1 | + | 59076 | 1.14 | 0.001369 |
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Target: 5'- aCGUCGUCCCGAAGAUGGAACUGUGGCa -3' miRNA: 3'- -GCAGCAGGGCUUCUACCUUGACACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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