Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19297 | 5' | -52.5 | NC_004685.1 | + | 33581 | 0.75 | 0.447803 |
Target: 5'- cGGcGUGGUCAccgUGGGACGAGGccGCGGUg -3' miRNA: 3'- -CCuCGCCAGU---ACCUUGCUCU--UGUCGa -5' |
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19297 | 5' | -52.5 | NC_004685.1 | + | 30964 | 0.77 | 0.331173 |
Target: 5'- uGAGCGGUCcUGGAugGAGucgaugcGCAGCc -3' miRNA: 3'- cCUCGCCAGuACCUugCUCu------UGUCGa -5' |
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19297 | 5' | -52.5 | NC_004685.1 | + | 53180 | 1.11 | 0.001997 |
Target: 5'- uGGAGCGGUCAUGGAACGAGAACAGCUg -3' miRNA: 3'- -CCUCGCCAGUACCUUGCUCUUGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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