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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19302 | 3' | -54.7 | NC_004685.1 | + | 10034 | 0.66 | 0.800005 |
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Target: 5'- gCUGGCcaacaaCUUGggGCCGUgcguggugcccgaGGAGGCGg -3' miRNA: 3'- aGACCGa-----GAGCuaCGGCAa------------CUUCCGC- -5' |
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| 19302 | 3' | -54.7 | NC_004685.1 | + | 25733 | 0.66 | 0.774659 |
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Target: 5'- --aGGCgcaCUCGAUGCCGaUGGuguacugguuGGCGu -3' miRNA: 3'- agaCCGa--GAGCUACGGCaACUu---------CCGC- -5' |
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| 19302 | 3' | -54.7 | NC_004685.1 | + | 22415 | 0.67 | 0.764641 |
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Target: 5'- aCUGGCgguuggCGGUGCUGUcGAuGGUGg -3' miRNA: 3'- aGACCGaga---GCUACGGCAaCUuCCGC- -5' |
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| 19302 | 3' | -54.7 | NC_004685.1 | + | 31164 | 0.67 | 0.744214 |
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Target: 5'- -aUGGaCUCuUCGAUGauGUcGAAGGCGa -3' miRNA: 3'- agACC-GAG-AGCUACggCAaCUUCCGC- -5' |
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| 19302 | 3' | -54.7 | NC_004685.1 | + | 62708 | 0.67 | 0.733829 |
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Target: 5'- -aUGG-UCUCGGUGUCGUUGucagagacgacGAGGCc -3' miRNA: 3'- agACCgAGAGCUACGGCAAC-----------UUCCGc -5' |
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| 19302 | 3' | -54.7 | NC_004685.1 | + | 6012 | 0.68 | 0.658946 |
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Target: 5'- --aGGCgucCUCGAUGCCGcgGcGGGGUGa -3' miRNA: 3'- agaCCGa--GAGCUACGGCaaC-UUCCGC- -5' |
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| 19302 | 3' | -54.7 | NC_004685.1 | + | 14219 | 0.72 | 0.42912 |
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Target: 5'- --cGGCUgUCGGUGUCGUcggaGAAGGUGa -3' miRNA: 3'- agaCCGAgAGCUACGGCAa---CUUCCGC- -5' |
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| 19302 | 3' | -54.7 | NC_004685.1 | + | 49912 | 1.09 | 0.001588 |
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Target: 5'- uUCUGGCUCUCGAUGCCGUUGAAGGCGc -3' miRNA: 3'- -AGACCGAGAGCUACGGCAACUUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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