Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19308 | 3' | -59.3 | NC_004685.1 | + | 46976 | 0.67 | 0.511366 |
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Target: 5'- cGG-CgGUGGCCUCGUCCacgccgaccgCGGUGa-- -3' miRNA: 3'- -UCaGgCGCCGGAGCAGGa---------GCCACauu -5' |
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| 19308 | 3' | -59.3 | NC_004685.1 | + | 26451 | 0.67 | 0.481504 |
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Target: 5'- uAG-CCGCGcGCCUUGgCCUCGGUc--- -3' miRNA: 3'- -UCaGGCGC-CGGAGCaGGAGCCAcauu -5' |
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| 19308 | 3' | -59.3 | NC_004685.1 | + | 53568 | 0.67 | 0.471738 |
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Target: 5'- gGGaCCGUGGCCgugcgCGUCggCGGUGUu- -3' miRNA: 3'- -UCaGGCGCCGGa----GCAGgaGCCACAuu -5' |
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| 19308 | 3' | -59.3 | NC_004685.1 | + | 55045 | 0.67 | 0.471738 |
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Target: 5'- --aCCGUGGUCUCGUcgaucaccucgcCCUCGGcguUGUGAa -3' miRNA: 3'- ucaGGCGCCGGAGCA------------GGAGCC---ACAUU- -5' |
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| 19308 | 3' | -59.3 | NC_004685.1 | + | 764 | 0.68 | 0.452514 |
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Target: 5'- cGGUCUuggcggcagcaGCGGCCUgaUCCUCGGcgGUGAu -3' miRNA: 3'- -UCAGG-----------CGCCGGAgcAGGAGCCa-CAUU- -5' |
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| 19308 | 3' | -59.3 | NC_004685.1 | + | 59725 | 0.69 | 0.371726 |
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Target: 5'- cGUCgGuCGGCCagcccggcaCGUCCUCGGUGa-- -3' miRNA: 3'- uCAGgC-GCCGGa--------GCAGGAGCCACauu -5' |
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| 19308 | 3' | -59.3 | NC_004685.1 | + | 58442 | 0.7 | 0.33129 |
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Target: 5'- --cCCGCGGCgUgcaggaUGUCCUCGGUGg-- -3' miRNA: 3'- ucaGGCGCCGgA------GCAGGAGCCACauu -5' |
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| 19308 | 3' | -59.3 | NC_004685.1 | + | 49339 | 0.75 | 0.168733 |
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Target: 5'- cAGcUCCGaCGGCagcagugugUCGUCCUCGGUGUAc -3' miRNA: 3'- -UC-AGGC-GCCGg--------AGCAGGAGCCACAUu -5' |
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| 19308 | 3' | -59.3 | NC_004685.1 | + | 36098 | 0.79 | 0.079151 |
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Target: 5'- --aCCGCGGCCUCGUCCcaCGGUGa-- -3' miRNA: 3'- ucaGGCGCCGGAGCAGGa-GCCACauu -5' |
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| 19308 | 3' | -59.3 | NC_004685.1 | + | 47291 | 1.05 | 0.000974 |
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Target: 5'- cAGUCCGCGGCCUCGUCCUCGGUGUAAc -3' miRNA: 3'- -UCAGGCGCCGGAGCAGGAGCCACAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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