Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19316 | 3' | -55.2 | NC_004685.1 | + | 46748 | 0.66 | 0.803782 |
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Target: 5'- aCUGCUGCUgcUGGGGGUGcuGGccGUCa- -3' miRNA: 3'- -GGCGACGA--GCCCCUACu-UCuaCAGga -5' |
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| 19316 | 3' | -55.2 | NC_004685.1 | + | 41435 | 0.66 | 0.803782 |
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Target: 5'- gCCGCUGC-CGGuugacggugcGGGUGAgcuGGGUGaauUCCUu -3' miRNA: 3'- -GGCGACGaGCC----------CCUACU---UCUAC---AGGA- -5' |
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| 19316 | 3' | -55.2 | NC_004685.1 | + | 12290 | 0.66 | 0.794311 |
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Target: 5'- gCCGCcGCgacCGGGGAgaaugUGAAGAucauUGUCa- -3' miRNA: 3'- -GGCGaCGa--GCCCCU-----ACUUCU----ACAGga -5' |
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| 19316 | 3' | -55.2 | NC_004685.1 | + | 15046 | 0.66 | 0.784676 |
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Target: 5'- aCGCUGCUCGGuucgcaccGcGGUGAcgAGGUGgCCg -3' miRNA: 3'- gGCGACGAGCC--------C-CUACU--UCUACaGGa -5' |
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| 19316 | 3' | -55.2 | NC_004685.1 | + | 23984 | 0.69 | 0.595467 |
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Target: 5'- aCCGUUcCUCGGGGGUGAGcuUGcCCa -3' miRNA: 3'- -GGCGAcGAGCCCCUACUUcuACaGGa -5' |
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| 19316 | 3' | -55.2 | NC_004685.1 | + | 43409 | 0.69 | 0.58473 |
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Target: 5'- gCCGUUGUUCaGGGugagGggGGUGUCg- -3' miRNA: 3'- -GGCGACGAGcCCCua--CuuCUACAGga -5' |
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| 19316 | 3' | -55.2 | NC_004685.1 | + | 60948 | 0.7 | 0.552777 |
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Target: 5'- gUCGUcgaGCUCGGGGAUGccGAUgGUCUg -3' miRNA: 3'- -GGCGa--CGAGCCCCUACuuCUA-CAGGa -5' |
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| 19316 | 3' | -55.2 | NC_004685.1 | + | 5458 | 0.71 | 0.490676 |
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Target: 5'- uCCGCUGCgCGGGGAacuccUGGAcAUGaUCCg -3' miRNA: 3'- -GGCGACGaGCCCCU-----ACUUcUAC-AGGa -5' |
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| 19316 | 3' | -55.2 | NC_004685.1 | + | 43276 | 1.09 | 0.001454 |
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Target: 5'- gCCGCUGCUCGGGGAUGAAGAUGUCCUc -3' miRNA: 3'- -GGCGACGAGCCCCUACUUCUACAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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