Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19316 | 5' | -50.4 | NC_004685.1 | + | 34501 | 0.66 | 0.944901 |
Target: 5'- --cGGACUCgUAUCgagcagCGggUUGguGCg -3' miRNA: 3'- cauCCUGAG-GUAGa-----GCuuAACguCGa -5' |
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19316 | 5' | -50.4 | NC_004685.1 | + | 34236 | 0.67 | 0.928802 |
Target: 5'- -aAGGACUUCAUCUCGuuc-GCgucgGGCa -3' miRNA: 3'- caUCCUGAGGUAGAGCuuaaCG----UCGa -5' |
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19316 | 5' | -50.4 | NC_004685.1 | + | 49109 | 0.74 | 0.569749 |
Target: 5'- -aGGGACUCCuUCUCGc---GCAGCUc -3' miRNA: 3'- caUCCUGAGGuAGAGCuuaaCGUCGA- -5' |
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19316 | 5' | -50.4 | NC_004685.1 | + | 43312 | 1.09 | 0.003462 |
Target: 5'- cGUAGGACUCCAUCUCGAAUUGCAGCUg -3' miRNA: 3'- -CAUCCUGAGGUAGAGCUUAACGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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