Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19338 | 3' | -57.8 | NC_004685.1 | + | 56260 | 0.67 | 0.559489 |
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Target: 5'- gCUCaAGUUGGGagaaCCggUGCUGCGUCCGg -3' miRNA: 3'- aGGG-UCAACCCac--GG--ACGACGUAGGU- -5' |
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| 19338 | 3' | -57.8 | NC_004685.1 | + | 63712 | 0.67 | 0.548895 |
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Target: 5'- aCCUcGUUGGG-GuCCUGC-GCAUCUAc -3' miRNA: 3'- aGGGuCAACCCaC-GGACGaCGUAGGU- -5' |
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| 19338 | 3' | -57.8 | NC_004685.1 | + | 65271 | 0.67 | 0.538365 |
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Target: 5'- aCCCuuGUcggGGGUGUggCaGCUGCGUCCGu -3' miRNA: 3'- aGGGu-CAa--CCCACG--GaCGACGUAGGU- -5' |
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| 19338 | 3' | -57.8 | NC_004685.1 | + | 37077 | 0.67 | 0.516487 |
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Target: 5'- gCCCAGggGgcgggccGGUGCCggugUGCUGCGUgCGg -3' miRNA: 3'- aGGGUCaaC-------CCACGG----ACGACGUAgGU- -5' |
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| 19338 | 3' | -57.8 | NC_004685.1 | + | 4192 | 0.7 | 0.365691 |
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Target: 5'- -gCCAGUUGGGUGCCgaaCUGCuugagcgCCu -3' miRNA: 3'- agGGUCAACCCACGGac-GACGua-----GGu -5' |
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| 19338 | 3' | -57.8 | NC_004685.1 | + | 26192 | 1.08 | 0.000704 |
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Target: 5'- cUCCCAGUUGGGUGCCUGCUGCAUCCAc -3' miRNA: 3'- -AGGGUCAACCCACGGACGACGUAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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