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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1935 | 5' | -50.9 | NC_001347.2 | + | 44058 | 0.66 | 0.998006 |
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Target: 5'- -gCUGggGGuGGcgGCAGUgguaCUGCUGAUg -3' miRNA: 3'- gaGACuuUCuCCa-CGUCA----GGCGAUUG- -5' |
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| 1935 | 5' | -50.9 | NC_001347.2 | + | 130858 | 0.66 | 0.996116 |
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Target: 5'- ---------cGGUGCAGUCCGCcAACg -3' miRNA: 3'- gagacuuucuCCACGUCAGGCGaUUG- -5' |
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| 1935 | 5' | -50.9 | NC_001347.2 | + | 153620 | 0.66 | 0.996116 |
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Target: 5'- -cCUGGAcgugguGGGGGUGCAggcGUCCGaagAGCa -3' miRNA: 3'- gaGACUU------UCUCCACGU---CAGGCga-UUG- -5' |
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| 1935 | 5' | -50.9 | NC_001347.2 | + | 67949 | 0.67 | 0.995464 |
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Target: 5'- uUCUcGGAGGAGGgagGCGGccgCCGCUc-- -3' miRNA: 3'- gAGA-CUUUCUCCa--CGUCa--GGCGAuug -5' |
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| 1935 | 5' | -50.9 | NC_001347.2 | + | 56270 | 0.67 | 0.992957 |
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Target: 5'- gUCUGAAAaugagcucGGGGaGCGGgcuugcaCCGCUGACg -3' miRNA: 3'- gAGACUUU--------CUCCaCGUCa------GGCGAUUG- -5' |
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| 1935 | 5' | -50.9 | NC_001347.2 | + | 221316 | 0.69 | 0.982812 |
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Target: 5'- --gUGAGGGGGGUGCuguaGGUCUGCa--- -3' miRNA: 3'- gagACUUUCUCCACG----UCAGGCGauug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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