Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19352 | 5' | -60.4 | NC_004685.1 | + | 37389 | 0.72 | 0.214161 |
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Target: 5'- gCAGGGCcgcUGcGGucGCCGCGCCACCa--- -3' miRNA: 3'- aGUCCCG---AC-CC--CGGCGUGGUGGaaag -5' |
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| 19352 | 5' | -60.4 | NC_004685.1 | + | 32195 | 0.72 | 0.214161 |
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Target: 5'- cCAGGGCUGGGcggguucucccaGUCGaCGCCGCCg--- -3' miRNA: 3'- aGUCCCGACCC------------CGGC-GUGGUGGaaag -5' |
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| 19352 | 5' | -60.4 | NC_004685.1 | + | 1206 | 0.76 | 0.110688 |
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Target: 5'- gCGGGGCcagGGGGCCGCaucggacuggcgGCCGCCcaUCg -3' miRNA: 3'- aGUCCCGa--CCCCGGCG------------UGGUGGaaAG- -5' |
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| 19352 | 5' | -60.4 | NC_004685.1 | + | 29319 | 0.76 | 0.106846 |
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Target: 5'- --uGGGCUGGGGCCGaugaucggcaaucuCACCACCg--- -3' miRNA: 3'- aguCCCGACCCCGGC--------------GUGGUGGaaag -5' |
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| 19352 | 5' | -60.4 | NC_004685.1 | + | 18676 | 1.09 | 0.000359 |
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Target: 5'- aUCAGGGCUGGGGCCGCACCACCUUUCu -3' miRNA: 3'- -AGUCCCGACCCCGGCGUGGUGGAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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