Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19374 | 5' | -59.3 | NC_004685.1 | + | 16224 | 0.66 | 0.554026 |
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Target: 5'- -aGCUGUgcgaagacuGCGGcGAGGuAgUGCCGACc -3' miRNA: 3'- gaCGACGu--------CGCCuCUCC-UgACGGCUG- -5' |
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| 19374 | 5' | -59.3 | NC_004685.1 | + | 33872 | 0.66 | 0.543605 |
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Target: 5'- -cGCUGCu-CGGuGAGGACUaCCGGg -3' miRNA: 3'- gaCGACGucGCCuCUCCUGAcGGCUg -5' |
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| 19374 | 5' | -59.3 | NC_004685.1 | + | 46748 | 0.66 | 0.522963 |
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Target: 5'- aCUGCUGCuGCuGGGGGuGCUGgCCGuCa -3' miRNA: 3'- -GACGACGuCGcCUCUCcUGAC-GGCuG- -5' |
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| 19374 | 5' | -59.3 | NC_004685.1 | + | 26590 | 0.67 | 0.482649 |
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Target: 5'- -aGCUGCgcgccGGCGGcaAGucGGCgGCCGACg -3' miRNA: 3'- gaCGACG-----UCGCC--UCucCUGaCGGCUG- -5' |
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| 19374 | 5' | -59.3 | NC_004685.1 | + | 51109 | 0.7 | 0.308451 |
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Target: 5'- aUGCUGCcGCGGuggcccuugauGGGGGug-GCCGACa -3' miRNA: 3'- gACGACGuCGCC-----------UCUCCugaCGGCUG- -5' |
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| 19374 | 5' | -59.3 | NC_004685.1 | + | 49195 | 0.7 | 0.293917 |
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Target: 5'- -gGCUuCGGCGGuGAuGACUGCUGGCg -3' miRNA: 3'- gaCGAcGUCGCCuCUcCUGACGGCUG- -5' |
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| 19374 | 5' | -59.3 | NC_004685.1 | + | 37860 | 0.71 | 0.273126 |
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Target: 5'- cCUGCgGCAGCGGGaucGAcgccGACUGCCGGg -3' miRNA: 3'- -GACGaCGUCGCCU---CUc---CUGACGGCUg -5' |
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| 19374 | 5' | -59.3 | NC_004685.1 | + | 19166 | 0.73 | 0.21782 |
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Target: 5'- -gGCUGUGGgaGGAGAuggauggcuccgGGGCUGCCGGCc -3' miRNA: 3'- gaCGACGUCg-CCUCU------------CCUGACGGCUG- -5' |
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| 19374 | 5' | -59.3 | NC_004685.1 | + | 65368 | 0.73 | 0.206908 |
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Target: 5'- cCUGCgGCucGCGGAGAGGcCcGCCGAg -3' miRNA: 3'- -GACGaCGu-CGCCUCUCCuGaCGGCUg -5' |
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| 19374 | 5' | -59.3 | NC_004685.1 | + | 9150 | 1.09 | 0.000479 |
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Target: 5'- cCUGCUGCAGCGGAGAGGACUGCCGACu -3' miRNA: 3'- -GACGACGUCGCCUCUCCUGACGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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