Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19383 | 3' | -56.2 | NC_004685.1 | + | 51314 | 0.72 | 0.377811 |
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Target: 5'- uGUCAGCGUCGGUGUCUGUGAcGCCGcGg -3' miRNA: 3'- -UAGUUGCAGUCACGGGUGCU-CGGCuC- -5' |
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| 19383 | 3' | -56.2 | NC_004685.1 | + | 14413 | 0.73 | 0.336007 |
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Target: 5'- -gCAGCGUCAGcUGCgCGcCGAGgCCGAGg -3' miRNA: 3'- uaGUUGCAGUC-ACGgGU-GCUC-GGCUC- -5' |
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| 19383 | 3' | -56.2 | NC_004685.1 | + | 9852 | 0.73 | 0.328062 |
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Target: 5'- gAUC-GCGUCGGUGCagccgUCACGGGCCGGc -3' miRNA: 3'- -UAGuUGCAGUCACG-----GGUGCUCGGCUc -5' |
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| 19383 | 3' | -56.2 | NC_004685.1 | + | 43805 | 0.73 | 0.320256 |
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Target: 5'- gAUCAGCGUCGGccGCCCcgaccugcGCGGGCCGc- -3' miRNA: 3'- -UAGUUGCAGUCa-CGGG--------UGCUCGGCuc -5' |
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| 19383 | 3' | -56.2 | NC_004685.1 | + | 20312 | 0.73 | 0.320256 |
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Target: 5'- cGUCuAUGUCGauGUGCCCgACGGGCCGGa -3' miRNA: 3'- -UAGuUGCAGU--CACGGG-UGCUCGGCUc -5' |
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| 19383 | 3' | -56.2 | NC_004685.1 | + | 5342 | 0.94 | 0.011499 |
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Target: 5'- cAUCAACGUCAGcGUCCACGAGCCGAGg -3' miRNA: 3'- -UAGUUGCAGUCaCGGGUGCUCGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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