Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19383 | 5' | -58.6 | NC_004685.1 | + | 43903 | 0.69 | 0.435126 |
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Target: 5'- uGCCCGGcgacacGUG-UGGCUGugGCUGAa- -3' miRNA: 3'- cCGGGCC------CACaACCGACugUGGCUgu -5' |
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| 19383 | 5' | -58.6 | NC_004685.1 | + | 13079 | 0.69 | 0.435126 |
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Target: 5'- gGGCCauccGUGgaGGCagGACACCGACGa -3' miRNA: 3'- -CCGGgcc-CACaaCCGa-CUGUGGCUGU- -5' |
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| 19383 | 5' | -58.6 | NC_004685.1 | + | 59830 | 0.7 | 0.399027 |
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Target: 5'- cGGCCCG--UGacGGCUG-CACCGACGc -3' miRNA: 3'- -CCGGGCccACaaCCGACuGUGGCUGU- -5' |
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| 19383 | 5' | -58.6 | NC_004685.1 | + | 8421 | 0.7 | 0.390306 |
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Target: 5'- uGCCCGacGGUG-UGGUcGGCAUCGACGu -3' miRNA: 3'- cCGGGC--CCACaACCGaCUGUGGCUGU- -5' |
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| 19383 | 5' | -58.6 | NC_004685.1 | + | 4091 | 0.7 | 0.373243 |
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Target: 5'- cGGCCCu-GUGgccGCUGAUGCCGACAu -3' miRNA: 3'- -CCGGGccCACaacCGACUGUGGCUGU- -5' |
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| 19383 | 5' | -58.6 | NC_004685.1 | + | 8330 | 0.7 | 0.373243 |
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Target: 5'- uGGagaagaCGGGUGUccaugcgcaggUGGUgGACACCGACAg -3' miRNA: 3'- -CCgg----GCCCACA-----------ACCGaCUGUGGCUGU- -5' |
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| 19383 | 5' | -58.6 | NC_004685.1 | + | 93 | 0.7 | 0.356693 |
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Target: 5'- uGCCgGGGgccUGUUGGCgugGACGCCGuuGCu -3' miRNA: 3'- cCGGgCCC---ACAACCGa--CUGUGGC--UGu -5' |
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| 19383 | 5' | -58.6 | NC_004685.1 | + | 30821 | 0.71 | 0.317615 |
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Target: 5'- cGGUgaGGGUGUgggUGGCaccgcgGGCACCGACc -3' miRNA: 3'- -CCGggCCCACA---ACCGa-----CUGUGGCUGu -5' |
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| 19383 | 5' | -58.6 | NC_004685.1 | + | 5377 | 1.1 | 0.000566 |
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Target: 5'- cGGCCCGGGUGUUGGCUGACACCGACAc -3' miRNA: 3'- -CCGGGCCCACAACCGACUGUGGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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