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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19393 | 3' | -56.5 | NC_004685.1 | + | 11536 | 0.67 | 0.621684 |
Target: 5'- cGCUCaUGCgUCGgcgcugcgCUGCGCUGGGcaUCGu -3' miRNA: 3'- -UGAGaACG-AGCa-------GACGCGGCCCa-AGC- -5' |
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19393 | 3' | -56.5 | NC_004685.1 | + | 59969 | 0.71 | 0.418405 |
Target: 5'- gGCgCUgaggGCaCG-CUGCGCCGGGUUCu -3' miRNA: 3'- -UGaGAa---CGaGCaGACGCGGCCCAAGc -5' |
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19393 | 3' | -56.5 | NC_004685.1 | + | 267 | 1.09 | 0.000986 |
Target: 5'- gACUCUUGCUCGUCUGCGCCGGGUUCGg -3' miRNA: 3'- -UGAGAACGAGCAGACGCGGCCCAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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