Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19408 | 3' | -53.8 | NC_004685.1 | + | 14531 | 0.66 | 0.850502 |
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Target: 5'- ----cGCCgCGCUGgugcucGAcaUGAGCCCCGa -3' miRNA: 3'- gcuuaUGG-GCGACau----CU--ACUCGGGGU- -5' |
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| 19408 | 3' | -53.8 | NC_004685.1 | + | 26848 | 0.67 | 0.805415 |
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Target: 5'- aCGGccGCCCGCgGUGGGgagugGAGCgaggCCCAg -3' miRNA: 3'- -GCUuaUGGGCGaCAUCUa----CUCG----GGGU- -5' |
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| 19408 | 3' | -53.8 | NC_004685.1 | + | 58043 | 0.67 | 0.766037 |
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Target: 5'- gCGcAcgGCCCGCUGUGcccGAUgGAGCgCCGc -3' miRNA: 3'- -GC-UuaUGGGCGACAU---CUA-CUCGgGGU- -5' |
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| 19408 | 3' | -53.8 | NC_004685.1 | + | 13883 | 0.68 | 0.735025 |
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Target: 5'- uCGAGUACCCcgguguGCUGgccaucUGGAcGAGCCaCCAc -3' miRNA: 3'- -GCUUAUGGG------CGAC------AUCUaCUCGG-GGU- -5' |
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| 19408 | 3' | -53.8 | NC_004685.1 | + | 51474 | 0.68 | 0.703087 |
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Target: 5'- cCGAAggACCCGCcgGUgcAGGUG-GCCCUg -3' miRNA: 3'- -GCUUa-UGGGCGa-CA--UCUACuCGGGGu -5' |
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| 19408 | 3' | -53.8 | NC_004685.1 | + | 66975 | 0.71 | 0.56117 |
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Target: 5'- gGAcgACCCGCUGUGGGacuGCCgCAu -3' miRNA: 3'- gCUuaUGGGCGACAUCUacuCGGgGU- -5' |
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| 19408 | 3' | -53.8 | NC_004685.1 | + | 43855 | 0.8 | 0.178355 |
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Target: 5'- gGAGUugCCGgUGUGGugccacAUGAGCCCCAg -3' miRNA: 3'- gCUUAugGGCgACAUC------UACUCGGGGU- -5' |
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| 19408 | 3' | -53.8 | NC_004685.1 | + | 7074 | 1.08 | 0.001975 |
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Target: 5'- uCGAAUACCCGCUGUAGAUGAGCCCCAc -3' miRNA: 3'- -GCUUAUGGGCGACAUCUACUCGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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