Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19412 | 3' | -51.1 | NC_004685.1 | + | 31730 | 0.68 | 0.865679 |
Target: 5'- gGGggCGGGCAGAUCAaccCGGG-GUa -3' miRNA: 3'- aCCaaGUUCGUCUAGUacuGCCCaCA- -5' |
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19412 | 3' | -51.1 | NC_004685.1 | + | 52309 | 0.68 | 0.857311 |
Target: 5'- cGGUgCGcGCugAGGUCAUGuuCGGGUGUa -3' miRNA: 3'- aCCAaGUuCG--UCUAGUACu-GCCCACA- -5' |
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19412 | 3' | -51.1 | NC_004685.1 | + | 8834 | 0.68 | 0.848696 |
Target: 5'- gGGUUCGaugugcGGCAGGcggUCAccGCGGGUGa -3' miRNA: 3'- aCCAAGU------UCGUCU---AGUacUGCCCACa -5' |
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19412 | 3' | -51.1 | NC_004685.1 | + | 9606 | 0.68 | 0.848696 |
Target: 5'- cGGUUCGGGCgAGggCAUGGUGGaGUGc -3' miRNA: 3'- aCCAAGUUCG-UCuaGUACUGCC-CACa -5' |
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19412 | 3' | -51.1 | NC_004685.1 | + | 9153 | 1.07 | 0.00417 |
Target: 5'- cUGGUUCAAGCAGAUCAUGACGGGUGUu -3' miRNA: 3'- -ACCAAGUUCGUCUAGUACUGCCCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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