Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19415 | 5' | -56.4 | NC_004685.1 | + | 26857 | 0.66 | 0.703969 |
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Target: 5'- cGCGGuGGGGAGUggagCGAGGCCc-- -3' miRNA: 3'- aCGCC-CUCUUCAgguaGCUCCGGaua -5' |
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| 19415 | 5' | -56.4 | NC_004685.1 | + | 82 | 0.66 | 0.671704 |
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Target: 5'- -uCGGGGGggGgugCCG--GGGGCCUGUu -3' miRNA: 3'- acGCCCUCuuCa--GGUagCUCCGGAUA- -5' |
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| 19415 | 5' | -56.4 | NC_004685.1 | + | 19417 | 0.67 | 0.606457 |
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Target: 5'- aGCGaGAG-GGUCCgGUCGAuGGCCUGa -3' miRNA: 3'- aCGCcCUCuUCAGG-UAGCU-CCGGAUa -5' |
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| 19415 | 5' | -56.4 | NC_004685.1 | + | 21298 | 0.67 | 0.606457 |
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Target: 5'- gGCGaGGAGAAGUCgAUCGccuGCCg-- -3' miRNA: 3'- aCGC-CCUCUUCAGgUAGCuc-CGGaua -5' |
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| 19415 | 5' | -56.4 | NC_004685.1 | + | 53519 | 0.71 | 0.385045 |
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Target: 5'- cGUGGGGGuGGUaCAUCGAGGCUUu- -3' miRNA: 3'- aCGCCCUCuUCAgGUAGCUCCGGAua -5' |
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| 19415 | 5' | -56.4 | NC_004685.1 | + | 10257 | 1.05 | 0.001603 |
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Target: 5'- aUGCGGGAGAAGUCCAUCGAGGCCUAUc -3' miRNA: 3'- -ACGCCCUCUUCAGGUAGCUCCGGAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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