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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19426 | 3' | -46.4 | NC_004685.1 | + | 59993 | 0.66 | 0.998405 |
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Target: 5'- gCUGG-CGAUGGaagACGUGACGcuggCGcUGAg -3' miRNA: 3'- -GACCaGUUACU---UGCACUGUa---GCaACU- -5' |
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| 19426 | 3' | -46.4 | NC_004685.1 | + | 41414 | 0.67 | 0.995952 |
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Target: 5'- gCUGaccgCAGUGAACGUGACGUuCGcUGc -3' miRNA: 3'- -GACca--GUUACUUGCACUGUA-GCaACu -5' |
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| 19426 | 3' | -46.4 | NC_004685.1 | + | 21338 | 0.67 | 0.995204 |
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Target: 5'- -cGG-CAAUGccaccgccgacAACGUGGCGUCGgcGAu -3' miRNA: 3'- gaCCaGUUAC-----------UUGCACUGUAGCaaCU- -5' |
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| 19426 | 3' | -46.4 | NC_004685.1 | + | 29374 | 0.67 | 0.994348 |
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Target: 5'- -cGGUCGAUGAAg--GGCGUCGgccgGAu -3' miRNA: 3'- gaCCAGUUACUUgcaCUGUAGCaa--CU- -5' |
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| 19426 | 3' | -46.4 | NC_004685.1 | + | 53884 | 0.68 | 0.98962 |
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Target: 5'- -gGGUCAGUGGucaGCGaggucgGGCAUCGUgGGg -3' miRNA: 3'- gaCCAGUUACU---UGCa-----CUGUAGCAaCU- -5' |
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| 19426 | 3' | -46.4 | NC_004685.1 | + | 65581 | 0.68 | 0.988054 |
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Target: 5'- -aGGUCcAUGccgGACGUGuCGUUGUUGGc -3' miRNA: 3'- gaCCAGuUAC---UUGCACuGUAGCAACU- -5' |
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| 19426 | 3' | -46.4 | NC_004685.1 | + | 59195 | 0.68 | 0.986312 |
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Target: 5'- cCUGGUCGAUGAGcCGUuucuucucggcGGCgAUCGcUGAg -3' miRNA: 3'- -GACCAGUUACUU-GCA-----------CUG-UAGCaACU- -5' |
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| 19426 | 3' | -46.4 | NC_004685.1 | + | 46978 | 0.69 | 0.984381 |
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Target: 5'- cCUGGUCAu---ACGUGACAUCcgaagGAu -3' miRNA: 3'- -GACCAGUuacuUGCACUGUAGcaa--CU- -5' |
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| 19426 | 3' | -46.4 | NC_004685.1 | + | 2799 | 0.7 | 0.960818 |
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Target: 5'- gUGGUgAAcucgucgcacuUGAGCGUGGCGUCGagggUGGg -3' miRNA: 3'- gACCAgUU-----------ACUUGCACUGUAGCa---ACU- -5' |
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| 19426 | 3' | -46.4 | NC_004685.1 | + | 14675 | 1.1 | 0.009967 |
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Target: 5'- gCUGGUCAAUGAACGUGACAUCGUUGAc -3' miRNA: 3'- -GACCAGUUACUUGCACUGUAGCAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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