miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
19452 5' -59 NC_004685.1 + 35531 0.66 0.585556
Target:  5'- aGCAGCUGGGcGUcgaCUaCGUGGGGccGUg -3'
miRNA:   3'- -CGUCGGCCC-CGaaaGA-GCACCCCa-CA- -5'
19452 5' -59 NC_004685.1 + 59920 0.66 0.56461
Target:  5'- cCAGCUGacugcGGGUcUUCUUG-GGGGUGUu -3'
miRNA:   3'- cGUCGGC-----CCCGaAAGAGCaCCCCACA- -5'
19452 5' -59 NC_004685.1 + 49743 0.67 0.513245
Target:  5'- --cGCCGGGGCggaUCaUCGcGGGGUu- -3'
miRNA:   3'- cguCGGCCCCGaa-AG-AGCaCCCCAca -5'
19452 5' -59 NC_004685.1 + 33737 0.69 0.381884
Target:  5'- cGCAGCCGGauGCcgUUCUCaccgGGGGUGc -3'
miRNA:   3'- -CGUCGGCCc-CGa-AAGAGca--CCCCACa -5'
19452 5' -59 NC_004685.1 + 25222 0.7 0.348665
Target:  5'- aGCAGaCCGGGGCUUUCcUGUaGGGc-- -3'
miRNA:   3'- -CGUC-GGCCCCGAAAGaGCAcCCCaca -5'
19452 5' -59 NC_004685.1 + 47369 0.73 0.242955
Target:  5'- cGCAGaCCGaGGuGCUgcguacgCUCGUGcGGGUGUa -3'
miRNA:   3'- -CGUC-GGC-CC-CGAaa-----GAGCAC-CCCACA- -5'
19452 5' -59 NC_004685.1 + 25159 1.08 0.000676
Target:  5'- gGCAGCCGGGGCUUUCUCGUGGGGUGUu -3'
miRNA:   3'- -CGUCGGCCCCGAAAGAGCACCCCACA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.