Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
1946 | 3' | -49.4 | NC_001347.2 | + | 171741 | 0.66 | 0.999376 |
Target: 5'- -uGGggCAAcGCGCGCUUGgacGCGCUa- -3' miRNA: 3'- guUCuaGUU-CGUGUGAACa--CGCGAgu -5' |
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1946 | 3' | -49.4 | NC_001347.2 | + | 167596 | 0.66 | 0.999231 |
Target: 5'- -uGGAUCAGcGC-CAUUUGUGCGUa-- -3' miRNA: 3'- guUCUAGUU-CGuGUGAACACGCGagu -5' |
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1946 | 3' | -49.4 | NC_001347.2 | + | 175081 | 0.66 | 0.999057 |
Target: 5'- cCGAGAcgcggCAGGCGCACUUucugGCGCg-- -3' miRNA: 3'- -GUUCUa----GUUCGUGUGAAca--CGCGagu -5' |
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1946 | 3' | -49.4 | NC_001347.2 | + | 153758 | 0.67 | 0.998608 |
Target: 5'- -cGGGUCGGGuCGCGCcaGUGCGCcgUCGa -3' miRNA: 3'- guUCUAGUUC-GUGUGaaCACGCG--AGU- -5' |
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1946 | 3' | -49.4 | NC_001347.2 | + | 98848 | 0.68 | 0.996046 |
Target: 5'- --uGGUCcAGUACAcCUUGcUGCGCUCc -3' miRNA: 3'- guuCUAGuUCGUGU-GAAC-ACGCGAGu -5' |
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1946 | 3' | -49.4 | NC_001347.2 | + | 161855 | 0.69 | 0.992781 |
Target: 5'- cCAGcAUCcAGCGCAC-UGUGCGUUCc -3' miRNA: 3'- -GUUcUAGuUCGUGUGaACACGCGAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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