Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19505 | 5' | -54.5 | NC_004685.1 | + | 36408 | 0.66 | 0.834837 |
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Target: 5'- aGCUGCGccagaACGCaUCGUGCGacuugaacggccaGGUGaAUGu -3' miRNA: 3'- -CGACGCa----UGCG-AGCACGC-------------CCACaUAC- -5' |
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| 19505 | 5' | -54.5 | NC_004685.1 | + | 42441 | 0.69 | 0.632102 |
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Target: 5'- gGCgGCGUACGacaacUGUGCGGGUGc--- -3' miRNA: 3'- -CGaCGCAUGCga---GCACGCCCACauac -5' |
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| 19505 | 5' | -54.5 | NC_004685.1 | + | 33749 | 0.71 | 0.555421 |
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Target: 5'- cGCUGCccggcgagacgugGUGCG-UCGUcGCGGGUGUcgGc -3' miRNA: 3'- -CGACG-------------CAUGCgAGCA-CGCCCACAuaC- -5' |
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| 19505 | 5' | -54.5 | NC_004685.1 | + | 68356 | 0.73 | 0.425127 |
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Target: 5'- -gUGCGUGCGC-CGcGCGGGUccgaGUGUGc -3' miRNA: 3'- cgACGCAUGCGaGCaCGCCCA----CAUAC- -5' |
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| 19505 | 5' | -54.5 | NC_004685.1 | + | 53792 | 0.81 | 0.136162 |
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Target: 5'- cGCUGCGUACugacacgccaGCUCGU-CGGGUGUGUa -3' miRNA: 3'- -CGACGCAUG----------CGAGCAcGCCCACAUAc -5' |
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| 19505 | 5' | -54.5 | NC_004685.1 | + | 47381 | 1.11 | 0.001253 |
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Target: 5'- uGCUGCGUACGCUCGUGCGGGUGUAUGa -3' miRNA: 3'- -CGACGCAUGCGAGCACGCCCACAUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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