Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19524 | 5' | -62.3 | NC_004685.1 | + | 53352 | 0.66 | 0.428528 |
Target: 5'- cUCCaGCGCgGCGccGGUggccgcgGCGAUGCUGg -3' miRNA: 3'- -AGGgCGCGaCGCucCCA-------CGCUGCGAC- -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 16974 | 0.66 | 0.420471 |
Target: 5'- gCCCG-GCcgGCGAGGGUGgGcagaccugcCGCUGc -3' miRNA: 3'- aGGGCgCGa-CGCUCCCACgCu--------GCGAC- -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 33742 | 0.66 | 0.39429 |
Target: 5'- aUCCCGcCGCUgcccgGCGAGacguGGUGCGucguCGCg- -3' miRNA: 3'- -AGGGC-GCGA-----CGCUC----CCACGCu---GCGac -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 37499 | 0.67 | 0.345204 |
Target: 5'- aCCCGCccaGCccugGCGA-GGUGUGGCGCg- -3' miRNA: 3'- aGGGCG---CGa---CGCUcCCACGCUGCGac -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 538 | 0.68 | 0.322358 |
Target: 5'- gCCCGCcaGCagcGCGcGGGUGuCGACGCa- -3' miRNA: 3'- aGGGCG--CGa--CGCuCCCAC-GCUGCGac -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 35212 | 0.69 | 0.293683 |
Target: 5'- gUCgCGCGCacggUGCGGauGGagacGUGCGGCGCUGu -3' miRNA: 3'- -AGgGCGCG----ACGCU--CC----CACGCUGCGAC- -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 4211 | 0.69 | 0.293683 |
Target: 5'- --gCGUGCgGCGAGGaGUGCGACGaCa- -3' miRNA: 3'- aggGCGCGaCGCUCC-CACGCUGC-Gac -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 1078 | 0.7 | 0.236519 |
Target: 5'- gCCCGCGCaguccUGCGGGcGGaUGCGGgGCa- -3' miRNA: 3'- aGGGCGCG-----ACGCUC-CC-ACGCUgCGac -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 20425 | 0.7 | 0.219648 |
Target: 5'- -aCCGCcaagGCggGCGAGGuGUGCG-CGCUGa -3' miRNA: 3'- agGGCG----CGa-CGCUCC-CACGCuGCGAC- -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 10638 | 0.71 | 0.198761 |
Target: 5'- aCCUGaaGCUGCccaAGGGUGCGAUGCUc -3' miRNA: 3'- aGGGCg-CGACGc--UCCCACGCUGCGAc -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 31059 | 0.72 | 0.183783 |
Target: 5'- aCCUacgcgaaGCGCUcgGCGAcauGGGUGCGGCGCUc -3' miRNA: 3'- aGGG-------CGCGA--CGCU---CCCACGCUGCGAc -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 724 | 0.72 | 0.179625 |
Target: 5'- aUCCCGaCGCcgUGCGAGccgaGGUGCcGACGCa- -3' miRNA: 3'- -AGGGC-GCG--ACGCUC----CCACG-CUGCGac -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 25874 | 0.72 | 0.166364 |
Target: 5'- gCCCGCGCagGUcGGGGcgGcCGACGCUGa -3' miRNA: 3'- aGGGCGCGa-CGcUCCCa-C-GCUGCGAC- -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 16320 | 0.76 | 0.095832 |
Target: 5'- aUCCCGCGCUGgugaCGGGcGGUGCcACGCUc -3' miRNA: 3'- -AGGGCGCGAC----GCUC-CCACGcUGCGAc -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 14274 | 0.76 | 0.090831 |
Target: 5'- gUCUgGCGauggaaagcCUGacCGAGGGUGCGACGCUGg -3' miRNA: 3'- -AGGgCGC---------GAC--GCUCCCACGCUGCGAC- -5' |
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19524 | 5' | -62.3 | NC_004685.1 | + | 55665 | 1.08 | 0.000323 |
Target: 5'- cUCCCGCGCUGCGAGGGUGCGACGCUGg -3' miRNA: 3'- -AGGGCGCGACGCUCCCACGCUGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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