Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19528 | 3' | -53.7 | NC_004685.1 | + | 41704 | 0.66 | 0.852085 |
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Target: 5'- aCGgcGCGGAGcuUGUUCUUGGCc--- -3' miRNA: 3'- -GCaaCGCCUCucACGGGAACCGuuuc -5' |
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| 19528 | 3' | -53.7 | NC_004685.1 | + | 52396 | 0.66 | 0.834689 |
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Target: 5'- --aUGCGGGcacgcUGCCCUUGGCucAGa -3' miRNA: 3'- gcaACGCCUcuc--ACGGGAACCGuuUC- -5' |
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| 19528 | 3' | -53.7 | NC_004685.1 | + | 63224 | 0.67 | 0.807019 |
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Target: 5'- cCGUUGCGGccgccaGGGGUGCCg--GGCu--- -3' miRNA: 3'- -GCAACGCC------UCUCACGGgaaCCGuuuc -5' |
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| 19528 | 3' | -53.7 | NC_004685.1 | + | 24297 | 0.67 | 0.767602 |
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Target: 5'- --aUGUGGGccGGUGCCCUUcGCGAAGa -3' miRNA: 3'- gcaACGCCUc-UCACGGGAAcCGUUUC- -5' |
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| 19528 | 3' | -53.7 | NC_004685.1 | + | 27859 | 0.71 | 0.572958 |
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Target: 5'- ---cGCGGAGGGUcGCCCUcGGCc--- -3' miRNA: 3'- gcaaCGCCUCUCA-CGGGAaCCGuuuc -5' |
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| 19528 | 3' | -53.7 | NC_004685.1 | + | 56727 | 1.1 | 0.001619 |
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Target: 5'- gCGUUGCGGAGAGUGCCCUUGGCAAAGa -3' miRNA: 3'- -GCAACGCCUCUCACGGGAACCGUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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