Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19540 | 3' | -57.9 | NC_004685.1 | + | 49611 | 0.66 | 0.588616 |
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Target: 5'- cGGGCUGGcCGacgaacgccaGGUGGuCgAGGAACGCCc -3' miRNA: 3'- -UUCGACCaGC----------CCACU-G-UCCUUGCGGu -5' |
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| 19540 | 3' | -57.9 | NC_004685.1 | + | 49860 | 0.67 | 0.546107 |
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Target: 5'- cGGCccucagGGUCGGccGACAGGuagucGCGCCAc -3' miRNA: 3'- uUCGa-----CCAGCCcaCUGUCCu----UGCGGU- -5' |
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| 19540 | 3' | -57.9 | NC_004685.1 | + | 17095 | 0.68 | 0.484401 |
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Target: 5'- cGAGCUGGUCGaGUcGCAGcGGuucgGCGCCGa -3' miRNA: 3'- -UUCGACCAGCcCAcUGUC-CU----UGCGGU- -5' |
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| 19540 | 3' | -57.9 | NC_004685.1 | + | 3229 | 0.7 | 0.355535 |
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Target: 5'- gGGGCggGGUCGGGgucgGGCcGGAccaucgGCGCCGg -3' miRNA: 3'- -UUCGa-CCAGCCCa---CUGuCCU------UGCGGU- -5' |
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| 19540 | 3' | -57.9 | NC_004685.1 | + | 57656 | 0.71 | 0.339199 |
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Target: 5'- cGGGCUG--CGGGUGACAGuGAGCacgGCCAg -3' miRNA: 3'- -UUCGACcaGCCCACUGUC-CUUG---CGGU- -5' |
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| 19540 | 3' | -57.9 | NC_004685.1 | + | 3807 | 0.71 | 0.331238 |
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Target: 5'- -uGCUGGcucgcaucucgUCGGGcGACGGG-ACGCCGc -3' miRNA: 3'- uuCGACC-----------AGCCCaCUGUCCuUGCGGU- -5' |
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| 19540 | 3' | -57.9 | NC_004685.1 | + | 24037 | 0.71 | 0.315731 |
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Target: 5'- uGGC-GGUUGGGcGGCAGGuacACGCCGa -3' miRNA: 3'- uUCGaCCAGCCCaCUGUCCu--UGCGGU- -5' |
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| 19540 | 3' | -57.9 | NC_004685.1 | + | 28340 | 0.74 | 0.216791 |
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Target: 5'- cAGCUGGagCGGGUGACu--GACGCCAc -3' miRNA: 3'- uUCGACCa-GCCCACUGuccUUGCGGU- -5' |
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| 19540 | 3' | -57.9 | NC_004685.1 | + | 62478 | 1.06 | 0.001106 |
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Target: 5'- cAAGCUGGUCGGGUGACAGGAACGCCAc -3' miRNA: 3'- -UUCGACCAGCCCACUGUCCUUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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