Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19554 | 3' | -57.2 | NC_004686.1 | + | 25074 | 0.66 | 0.628459 |
Target: 5'- cGGGCGacaCGGGUuucGAGGUccgGCACGGGCGg- -3' miRNA: 3'- -CUUGC---GCCUA---CUCCG---CGUGCUCGCag -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 21900 | 0.66 | 0.606695 |
Target: 5'- -cGCGCcGccGAGGCGCACccgcaaguGCGUCg -3' miRNA: 3'- cuUGCGcCuaCUCCGCGUGcu------CGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 7325 | 0.66 | 0.59584 |
Target: 5'- cAAUGCGGAUGccGGUgaGCACacccgGGGCGUCc -3' miRNA: 3'- cUUGCGCCUACu-CCG--CGUG-----CUCGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 5408 | 0.66 | 0.57423 |
Target: 5'- cGGGCGUGGcUGAGGUGC-CG-GUGgUCa -3' miRNA: 3'- -CUUGCGCCuACUCCGCGuGCuCGC-AG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 21822 | 0.66 | 0.574229 |
Target: 5'- --uUGCGGGUGcgccucGGCgGCGCGGGCG-Cg -3' miRNA: 3'- cuuGCGCCUACu-----CCG-CGUGCUCGCaG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 19047 | 0.67 | 0.56349 |
Target: 5'- ---gGCGGGUcGGGUGCugGccggcaGGCGUCu -3' miRNA: 3'- cuugCGCCUAcUCCGCGugC------UCGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 47792 | 0.67 | 0.56349 |
Target: 5'- gGGAUGCGGAguuGGCGgcUACGGGCGc- -3' miRNA: 3'- -CUUGCGCCUacuCCGC--GUGCUCGCag -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 26506 | 0.67 | 0.56349 |
Target: 5'- cAGCGCaGGUGucGCGCAUGGagaugaucuguGCGUCg -3' miRNA: 3'- cUUGCGcCUACucCGCGUGCU-----------CGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 56180 | 0.67 | 0.55174 |
Target: 5'- -cGCGUuuggucuGGucaGAGGUGCACG-GCGUCg -3' miRNA: 3'- cuUGCG-------CCua-CUCCGCGUGCuCGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 26545 | 0.67 | 0.521147 |
Target: 5'- uGACGCGGGUGgcAGGCaGUuccaguuucagaGCGAGCG-Ca -3' miRNA: 3'- cUUGCGCCUAC--UCCG-CG------------UGCUCGCaG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 11771 | 0.67 | 0.518018 |
Target: 5'- aGAACGCGGGcagcgccggauccaUGuauGGgGCugGGGCGa- -3' miRNA: 3'- -CUUGCGCCU--------------ACu--CCgCGugCUCGCag -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 55050 | 0.68 | 0.480099 |
Target: 5'- uGGCGCGGuUGuAGGCGgCGCugcgguugaacuGGGCGUCu -3' miRNA: 3'- cUUGCGCCuAC-UCCGC-GUG------------CUCGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 52687 | 0.69 | 0.440695 |
Target: 5'- --uCGCGGgcGAGGCGaACGuuguggagGGCGUCg -3' miRNA: 3'- cuuGCGCCuaCUCCGCgUGC--------UCGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 45864 | 0.69 | 0.440695 |
Target: 5'- ---gGCGGAUGAaGUGUugG-GCGUCg -3' miRNA: 3'- cuugCGCCUACUcCGCGugCuCGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 17340 | 0.69 | 0.430183 |
Target: 5'- aGGAUGCGcuaGGGGCGCugGccaacugGGCGUCa -3' miRNA: 3'- -CUUGCGCcuaCUCCGCGugC-------UCGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 30559 | 0.69 | 0.40961 |
Target: 5'- cGAuCGCGGGUGAGGCauuCACGGccggugcgcaguauGCGUUg -3' miRNA: 3'- -CUuGCGCCUACUCCGc--GUGCU--------------CGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 48133 | 0.7 | 0.367776 |
Target: 5'- cGAGCGCGGAUGAuccGCGUguccuggagGCG-GUGUCg -3' miRNA: 3'- -CUUGCGCCUACUc--CGCG---------UGCuCGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 55870 | 0.7 | 0.350888 |
Target: 5'- cGAUGCGGuguUGguAGGCGUAgGGGCGUUg -3' miRNA: 3'- cUUGCGCCu--AC--UCCGCGUgCUCGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 28759 | 0.7 | 0.350887 |
Target: 5'- gGGGCGCGG-UGAuGCGCggguuuuaugcgGCGGGUGUCg -3' miRNA: 3'- -CUUGCGCCuACUcCGCG------------UGCUCGCAG- -5' |
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19554 | 3' | -57.2 | NC_004686.1 | + | 37035 | 0.72 | 0.268036 |
Target: 5'- gGGugGCuGGGUGGauccgugguuGGCGCGCcugGAGCGUCa -3' miRNA: 3'- -CUugCG-CCUACU----------CCGCGUG---CUCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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