Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19555 | 3' | -52.9 | NC_004686.1 | + | 48234 | 0.66 | 0.839582 |
Target: 5'- -gGGAAGCCGCuGCCcgGUCGGG-UCa -3' miRNA: 3'- agCUUUCGGUGuUGGaaCGGCCUgAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 49962 | 0.66 | 0.830493 |
Target: 5'- aUCGuuucGCCGCGGCCUUcgucGCCGGcgAgUCg -3' miRNA: 3'- -AGCuuu-CGGUGUUGGAA----CGGCC--UgAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 41334 | 0.66 | 0.830493 |
Target: 5'- -aGGAAGCCcgaGACCUcGCCGcuGCUCu -3' miRNA: 3'- agCUUUCGGug-UUGGAaCGGCc-UGAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 31104 | 0.66 | 0.811671 |
Target: 5'- aUUGAGAaGCUGCGACC--GCUGcGGCUCa -3' miRNA: 3'- -AGCUUU-CGGUGUUGGaaCGGC-CUGAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 34886 | 0.66 | 0.80196 |
Target: 5'- uUCGAGGGCCACAACg-UGCgGcACg- -3' miRNA: 3'- -AGCUUUCGGUGUUGgaACGgCcUGag -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 3749 | 0.66 | 0.80196 |
Target: 5'- -aGgcGGCUucCAACUcUGCUGGGCUCa -3' miRNA: 3'- agCuuUCGGu-GUUGGaACGGCCUGAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 55746 | 0.67 | 0.792063 |
Target: 5'- uUCGAGuaCCACAGCCcaGUCGGugUg -3' miRNA: 3'- -AGCUUucGGUGUUGGaaCGGCCugAg -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 23612 | 0.67 | 0.781992 |
Target: 5'- aCGAGGGCgACuuuCCaggUGUCGGAgUCg -3' miRNA: 3'- aGCUUUCGgUGuu-GGa--ACGGCCUgAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 4666 | 0.68 | 0.740203 |
Target: 5'- gCG-AAGCCGCAacgcuuccGCUUUGgCGGugUCc -3' miRNA: 3'- aGCuUUCGGUGU--------UGGAACgGCCugAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 5637 | 0.68 | 0.72944 |
Target: 5'- gUCGAcgcGGUCACAGCCU--CCGGGC-Cg -3' miRNA: 3'- -AGCUu--UCGGUGUUGGAacGGCCUGaG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 8178 | 0.68 | 0.718576 |
Target: 5'- aUCGAcuGCUACaAGCC-UGCgaaGGGCUCg -3' miRNA: 3'- -AGCUuuCGGUG-UUGGaACGg--CCUGAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 1157 | 0.69 | 0.663155 |
Target: 5'- cCGu-AGCCACcACCgggUGCUGGGCa- -3' miRNA: 3'- aGCuuUCGGUGuUGGa--ACGGCCUGag -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 5460 | 0.7 | 0.595754 |
Target: 5'- uUCGcAGGGCCGCAAgCUguacCCGGaACUCg -3' miRNA: 3'- -AGC-UUUCGGUGUUgGAac--GGCC-UGAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 49829 | 0.7 | 0.57344 |
Target: 5'- aUCGggGcaucGCCAUAACCcgcgugGCCGG-CUCu -3' miRNA: 3'- -AGCuuU----CGGUGUUGGaa----CGGCCuGAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 33726 | 0.72 | 0.501569 |
Target: 5'- aUGAGAGCCagcagaGCAGCCUUGUcgcgagcagagaguuCGGugUCg -3' miRNA: 3'- aGCUUUCGG------UGUUGGAACG---------------GCCugAG- -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 3700 | 0.72 | 0.485765 |
Target: 5'- aUCGAGAGCCAguaccguCAAcCCUUGCaGGACg- -3' miRNA: 3'- -AGCUUUCGGU-------GUU-GGAACGgCCUGag -5' |
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19555 | 3' | -52.9 | NC_004686.1 | + | 729 | 1.1 | 0.001472 |
Target: 5'- aUCGAAAGCCACAACCUUGCCGGACUCc -3' miRNA: 3'- -AGCUUUCGGUGUUGGAACGGCCUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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