Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19556 | 5' | -51.3 | NC_004686.1 | + | 24596 | 0.66 | 0.915155 |
Target: 5'- ---aGCCGGCGgggccgcgggaUUGAACGUggaucUUCAUGa -3' miRNA: 3'- agugCGGCCGCa----------AACUUGCG-----AAGUAC- -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 3357 | 0.66 | 0.908572 |
Target: 5'- -aGCGCCGGCGU--GAaaucauccaccGCGcCUUCAg- -3' miRNA: 3'- agUGCGGCCGCAaaCU-----------UGC-GAAGUac -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 30542 | 0.66 | 0.908572 |
Target: 5'- uUCACgGCCGGUGcgcaguaugcgUUGGAuCGCaUCGUGg -3' miRNA: 3'- -AGUG-CGGCCGCa----------AACUU-GCGaAGUAC- -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 866 | 0.66 | 0.901701 |
Target: 5'- gCACGCUGGCGUgugaugUGAugGgU--GUGa -3' miRNA: 3'- aGUGCGGCCGCAa-----ACUugCgAagUAC- -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 13886 | 0.66 | 0.901701 |
Target: 5'- gUCGCGCagcaucugcucuUGGCGUUUcuGCGCgagCGUGg -3' miRNA: 3'- -AGUGCG------------GCCGCAAAcuUGCGaa-GUAC- -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 1345 | 0.66 | 0.894546 |
Target: 5'- -aACGCCGGCGUgauAAgGCUUUg-- -3' miRNA: 3'- agUGCGGCCGCAaacUUgCGAAGuac -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 48020 | 0.66 | 0.894546 |
Target: 5'- gUCGCGCCGGCGagccaaccGAAC-CUUCc-- -3' miRNA: 3'- -AGUGCGGCCGCaaa-----CUUGcGAAGuac -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 36962 | 0.66 | 0.88711 |
Target: 5'- --gUGCCGGCGUgaaucuugUUGAGCGUUUgAa- -3' miRNA: 3'- aguGCGGCCGCA--------AACUUGCGAAgUac -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 20103 | 0.66 | 0.88711 |
Target: 5'- aCACGgCGGUGgccgUGAACgGUUUCGUc -3' miRNA: 3'- aGUGCgGCCGCaa--ACUUG-CGAAGUAc -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 2008 | 0.66 | 0.884826 |
Target: 5'- gUCGCGCCGGCaggaucguacuucgGgaggUUGGAUGCUU-GUGc -3' miRNA: 3'- -AGUGCGGCCG--------------Ca---AACUUGCGAAgUAC- -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 12067 | 0.67 | 0.8794 |
Target: 5'- -gGCGCCGGCGg--GAAUGUaucccucagUUCGUc -3' miRNA: 3'- agUGCGGCCGCaaaCUUGCG---------AAGUAc -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 32173 | 0.68 | 0.82775 |
Target: 5'- aCGCGUCGGCGaUUGAua-UUUCGUGg -3' miRNA: 3'- aGUGCGGCCGCaAACUugcGAAGUAC- -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 33405 | 0.68 | 0.808693 |
Target: 5'- -aACGCCGGUGUUgcUGugUGUUUCGUc -3' miRNA: 3'- agUGCGGCCGCAA--ACuuGCGAAGUAc -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 27276 | 0.69 | 0.778668 |
Target: 5'- cCACGCCGGUGUgguucUUGAACGg--CAa- -3' miRNA: 3'- aGUGCGGCCGCA-----AACUUGCgaaGUac -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 32164 | 0.69 | 0.768324 |
Target: 5'- -gGCGCCGGUGag-GAACGCcaUCAUc -3' miRNA: 3'- agUGCGGCCGCaaaCUUGCGa-AGUAc -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 33760 | 0.73 | 0.557452 |
Target: 5'- cCACGCCGGCGg--GAGCaCcgCAUGa -3' miRNA: 3'- aGUGCGGCCGCaaaCUUGcGaaGUAC- -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 9233 | 0.74 | 0.477568 |
Target: 5'- cUCACgGCCGGCGgcuugucagagGGACGCUUC-UGa -3' miRNA: 3'- -AGUG-CGGCCGCaaa--------CUUGCGAAGuAC- -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 3288 | 0.75 | 0.440776 |
Target: 5'- uUCACGCCGGCGcugcgGAAgGCgUUCAUu -3' miRNA: 3'- -AGUGCGGCCGCaaa--CUUgCG-AAGUAc -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 95 | 0.76 | 0.383231 |
Target: 5'- --uCGCCGGCGUgacggGGACGCUcCGUGu -3' miRNA: 3'- aguGCGGCCGCAaa---CUUGCGAaGUAC- -5' |
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19556 | 5' | -51.3 | NC_004686.1 | + | 1413 | 1.1 | 0.002151 |
Target: 5'- aUCACGCCGGCGUUUGAACGCUUCAUGa -3' miRNA: 3'- -AGUGCGGCCGCAAACUUGCGAAGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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