Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19557 | 5' | -59.7 | NC_004686.1 | + | 24045 | 0.66 | 0.481662 |
Target: 5'- cAGCCaggUACCCGgCCGacuCCCCgAGC-CCGa -3' miRNA: 3'- -UCGGa--AUGGGCaGGU---GGGG-UCGuGGU- -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 37957 | 0.66 | 0.471747 |
Target: 5'- gGGCCgaccUGCCCGU-CACCCgCAaCGCCc -3' miRNA: 3'- -UCGGa---AUGGGCAgGUGGG-GUcGUGGu -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 47967 | 0.66 | 0.464869 |
Target: 5'- aAGCCUcuacacgUACCUccguauguggaacguGUUCACCCCGGaggGCCAa -3' miRNA: 3'- -UCGGA-------AUGGG---------------CAGGUGGGGUCg--UGGU- -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 19274 | 0.67 | 0.405476 |
Target: 5'- cGCCcacACCCGU--ACCaCCAGCACCc -3' miRNA: 3'- uCGGaa-UGGGCAggUGG-GGUCGUGGu -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 11883 | 0.67 | 0.387643 |
Target: 5'- aAGUCguuggcGCCC-UUCGCCCCAGCcCCAu -3' miRNA: 3'- -UCGGaa----UGGGcAGGUGGGGUCGuGGU- -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 47908 | 0.68 | 0.370334 |
Target: 5'- cGCCgccGCUCaugacaUCCGCCCCcGCGCCAa -3' miRNA: 3'- uCGGaa-UGGGc-----AGGUGGGGuCGUGGU- -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 55583 | 0.68 | 0.36188 |
Target: 5'- cGCUgacUACCUGUCCAUCgacgaCGGCACCc -3' miRNA: 3'- uCGGa--AUGGGCAGGUGGg----GUCGUGGu -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 43680 | 0.68 | 0.361041 |
Target: 5'- cGCCUagACCCGuugaUCCGCCCaucauugCGGCGCUg -3' miRNA: 3'- uCGGAa-UGGGC----AGGUGGG-------GUCGUGGu -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 54454 | 0.7 | 0.277913 |
Target: 5'- uGCCcUGCCCGacgCCgaACUCCAGUGCCGu -3' miRNA: 3'- uCGGaAUGGGCa--GG--UGGGGUCGUGGU- -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 22729 | 0.7 | 0.264425 |
Target: 5'- cAGCUc-ACCCG-CCuaacCCCCGGCGCCGc -3' miRNA: 3'- -UCGGaaUGGGCaGGu---GGGGUCGUGGU- -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 33933 | 0.7 | 0.264425 |
Target: 5'- cGGCCUU-CgCGU-CACCCCGGC-CCAa -3' miRNA: 3'- -UCGGAAuGgGCAgGUGGGGUCGuGGU- -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 28246 | 0.7 | 0.257883 |
Target: 5'- cGCCgaACCCGgaguaCCACCCggaguGGCGCCAa -3' miRNA: 3'- uCGGaaUGGGCa----GGUGGGg----UCGUGGU- -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 21091 | 0.71 | 0.223094 |
Target: 5'- cGCCgcagcagcgaaacUACCCGUaCCGCCCCcaauaccagugaucGGCGCCGa -3' miRNA: 3'- uCGGa------------AUGGGCA-GGUGGGG--------------UCGUGGU- -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 54296 | 0.74 | 0.133698 |
Target: 5'- cAGCCUUGgUCG-CCGCCUCGGCAaCCAg -3' miRNA: 3'- -UCGGAAUgGGCaGGUGGGGUCGU-GGU- -5' |
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19557 | 5' | -59.7 | NC_004686.1 | + | 2456 | 1.08 | 0.000401 |
Target: 5'- cAGCCUUACCCGUCCACCCCAGCACCAg -3' miRNA: 3'- -UCGGAAUGGGCAGGUGGGGUCGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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