miRNA: 3'- uGUGGACGCGGccgUGUGGCAUuUGU- -5'
miRNA: 3'- uGUGGA---CgC-GG---CCGUGUGGCAUUUGU- -5'
miRNA: 3'- -UGUGGACGCGGCCGUGUGGCAUUUGU- -5'
miRNA: 3'- -UGUGGACgCGGCCGU-GUGGCa----UUUGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19558 | 5' | -55.7 | NC_004686.1 | + | 29875 | 0.7 | 0.444782 |
|
Target: 5'- gUACCUGCGCC---GCACCGUGuACGg -3' miRNA: 3'- uGUGGACGCGGccgUGUGGCAUuUGU- -5' |
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| 19558 | 5' | -55.7 | NC_004686.1 | + | 32568 | 0.7 | 0.415931 |
|
Target: 5'- cCACCUaccGgGaCUacgGGCACACCGUGAACAa -3' miRNA: 3'- uGUGGA---CgC-GG---CCGUGUGGCAUUUGU- -5' |
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| 19558 | 5' | -55.7 | NC_004686.1 | + | 2874 | 1.08 | 0.001015 |
|
Target: 5'- aACACCUGCGCCGGCACACCGUAAACAu -3' miRNA: 3'- -UGUGGACGCGGCCGUGUGGCAUUUGU- -5' |
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| 19558 | 5' | -55.7 | NC_004686.1 | + | 32782 | 0.66 | 0.668795 |
|
Target: 5'- gGCAUCUGgGUCGGCAgCAUCGaauugGGAUAc -3' miRNA: 3'- -UGUGGACgCGGCCGU-GUGGCa----UUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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