Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19559 | 3' | -53 | NC_004686.1 | + | 42396 | 0.66 | 0.784125 |
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Target: 5'- -uGCcGgGGAA---CCGCAUCAGCAc -3' miRNA: 3'- uuCGaCgCCUUauaGGCGUAGUCGUa -5' |
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| 19559 | 3' | -53 | NC_004686.1 | + | 20075 | 0.66 | 0.784125 |
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Target: 5'- -cGCUGaaccgaucCGGAaaugcAUAUCCgGCGUCAGCGg -3' miRNA: 3'- uuCGAC--------GCCU-----UAUAGG-CGUAGUCGUa -5' |
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| 19559 | 3' | -53 | NC_004686.1 | + | 45352 | 0.66 | 0.773655 |
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Target: 5'- aGAGCggguucaaUGCGGAAuggucagugcUAUCCGCGUguGCc- -3' miRNA: 3'- -UUCG--------ACGCCUU----------AUAGGCGUAguCGua -5' |
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| 19559 | 3' | -53 | NC_004686.1 | + | 35274 | 0.67 | 0.719159 |
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Target: 5'- gGAGUUGuCGGu---UCCgGCGUCGGCGUg -3' miRNA: 3'- -UUCGAC-GCCuuauAGG-CGUAGUCGUA- -5' |
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| 19559 | 3' | -53 | NC_004686.1 | + | 35259 | 0.74 | 0.303706 |
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Target: 5'- cAGGCaGCGcAAUAUCCGUAUCGGCGg -3' miRNA: 3'- -UUCGaCGCcUUAUAGGCGUAGUCGUa -5' |
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| 19559 | 3' | -53 | NC_004686.1 | + | 3137 | 1.03 | 0.003066 |
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Target: 5'- cAAGCUGCGGAAUAUCCGCAUCAGCAUc -3' miRNA: 3'- -UUCGACGCCUUAUAGGCGUAGUCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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