Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19559 | 5' | -61.5 | NC_004686.1 | + | 25816 | 0.66 | 0.426683 |
Target: 5'- uGGCUg-UGcgCCGGCCCGcGGcCGGGa -3' miRNA: 3'- uCUGGgaACuaGGCCGGGU-CC-GCCCg -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 11984 | 0.66 | 0.426683 |
Target: 5'- cGGCaCC--GA-CCGGCCCAGGa-GGCa -3' miRNA: 3'- uCUG-GGaaCUaGGCCGGGUCCgcCCG- -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 3191 | 0.66 | 0.408614 |
Target: 5'- cAGACCaaUGcgcAUCCgGGCUCGcGGCGGGg -3' miRNA: 3'- -UCUGGgaAC---UAGG-CCGGGU-CCGCCCg -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 37743 | 0.66 | 0.407723 |
Target: 5'- --uCCCagGAUCCGGCcaguguccccauaCCAGGCGGu- -3' miRNA: 3'- ucuGGGaaCUAGGCCG-------------GGUCCGCCcg -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 19031 | 0.66 | 0.39976 |
Target: 5'- uGACCU---GUUCGGUgUGGGCGGGUc -3' miRNA: 3'- uCUGGGaacUAGGCCGgGUCCGCCCG- -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 30222 | 0.66 | 0.39103 |
Target: 5'- uGACCCUUGAcgUGGCCaauGGUcgauGGGUg -3' miRNA: 3'- uCUGGGAACUagGCCGGgu-CCG----CCCG- -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 23932 | 0.67 | 0.34133 |
Target: 5'- -aACCCUUGAcgCGGCCgacaAGGCaucGGGCu -3' miRNA: 3'- ucUGGGAACUagGCCGGg---UCCG---CCCG- -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 3835 | 0.68 | 0.318248 |
Target: 5'- gGGGCCUacGcgCUGG-CCGGGUGGGUg -3' miRNA: 3'- -UCUGGGaaCuaGGCCgGGUCCGCCCG- -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 25910 | 0.69 | 0.262512 |
Target: 5'- -aACCgUUGAUCUGuCCCGGccGCGGGCc -3' miRNA: 3'- ucUGGgAACUAGGCcGGGUC--CGCCCG- -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 26446 | 0.7 | 0.220489 |
Target: 5'- -aGCCCUUGggCaGGUCgGGGCGGGg -3' miRNA: 3'- ucUGGGAACuaGgCCGGgUCCGCCCg -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 25064 | 0.7 | 0.220489 |
Target: 5'- gGGuuUCgaGGUCCGGCaCGGGCGGGUu -3' miRNA: 3'- -UCugGGaaCUAGGCCGgGUCCGCCCG- -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 39708 | 0.71 | 0.189163 |
Target: 5'- cGACCCcaucccUGAacaugUCCuGGCCCAGGCGGcccGCc -3' miRNA: 3'- uCUGGGa-----ACU-----AGG-CCGGGUCCGCC---CG- -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 43676 | 0.71 | 0.189163 |
Target: 5'- uAGACCCgUUGAUCC-GCCCAucauuGCGGcGCu -3' miRNA: 3'- -UCUGGG-AACUAGGcCGGGUc----CGCC-CG- -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 11380 | 0.72 | 0.170513 |
Target: 5'- cAGGCCCUucUGGUgggguuguuuccUCGGUUCGGGuCGGGCg -3' miRNA: 3'- -UCUGGGA--ACUA------------GGCCGGGUCC-GCCCG- -5' |
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19559 | 5' | -61.5 | NC_004686.1 | + | 3103 | 1.1 | 0.000202 |
Target: 5'- gAGACCCUUGAUCCGGCCCAGGCGGGCu -3' miRNA: 3'- -UCUGGGAACUAGGCCGGGUCCGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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