Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19560 | 3' | -49.8 | NC_004686.1 | + | 37423 | 0.71 | 0.737757 |
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Target: 5'- aUCGCCGAAGCGCGcuacacGugGCCg-- -3' miRNA: 3'- -AGCGGUUUCGUGCuuac--UugCGGaag -5' |
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| 19560 | 3' | -49.8 | NC_004686.1 | + | 1426 | 0.72 | 0.68167 |
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Target: 5'- gCGCCAAAGCcuuaucacgccgGCGuuUGAACG-CUUCa -3' miRNA: 3'- aGCGGUUUCG------------UGCuuACUUGCgGAAG- -5' |
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| 19560 | 3' | -49.8 | NC_004686.1 | + | 9448 | 0.72 | 0.658783 |
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Target: 5'- cCGCCAAGGCugGugcaGACGCCg-- -3' miRNA: 3'- aGCGGUUUCGugCuuacUUGCGGaag -5' |
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| 19560 | 3' | -49.8 | NC_004686.1 | + | 17348 | 0.73 | 0.635789 |
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Target: 5'- aUCGCCGugaucaccGCACGggUGAacucgACGCCcUCc -3' miRNA: 3'- -AGCGGUuu------CGUGCuuACU-----UGCGGaAG- -5' |
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| 19560 | 3' | -49.8 | NC_004686.1 | + | 11092 | 0.74 | 0.57729 |
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Target: 5'- cCGCCGuGAGCACGAcccccaccgaaacGUGGACGCUUcCa -3' miRNA: 3'- aGCGGU-UUCGUGCU-------------UACUUGCGGAaG- -5' |
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| 19560 | 3' | -49.8 | NC_004686.1 | + | 703 | 0.76 | 0.462042 |
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Target: 5'- cCGUCGGAGC-CGAcuccgacucagguuUGAGCGCCUUCa -3' miRNA: 3'- aGCGGUUUCGuGCUu-------------ACUUGCGGAAG- -5' |
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| 19560 | 3' | -49.8 | NC_004686.1 | + | 49411 | 0.8 | 0.271964 |
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Target: 5'- uUCGCCA-AGCGCGu-UGAGCGCgUUCg -3' miRNA: 3'- -AGCGGUuUCGUGCuuACUUGCGgAAG- -5' |
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| 19560 | 3' | -49.8 | NC_004686.1 | + | 3386 | 0.96 | 0.025646 |
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Target: 5'- aUCG-CAAAGCACGAAUGAACGCCUUCc -3' miRNA: 3'- -AGCgGUUUCGUGCUUACUUGCGGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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