Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19561 | 5' | -58.4 | NC_004686.1 | + | 14625 | 0.66 | 0.526795 |
Target: 5'- gGCCCGCAccgUC-GCCgaguuCGCCGACg-- -3' miRNA: 3'- aUGGGCGU---AGuCGGac---GCGGCUGaag -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 17710 | 0.66 | 0.516293 |
Target: 5'- uUGCCCGCAUCaauagaccAGCCgGCGUC-ACUc- -3' miRNA: 3'- -AUGGGCGUAG--------UCGGaCGCGGcUGAag -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 12231 | 0.66 | 0.505876 |
Target: 5'- gGCCC-CG-CGGCCUGacCGCCGGCg-- -3' miRNA: 3'- aUGGGcGUaGUCGGAC--GCGGCUGaag -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 30047 | 0.66 | 0.483288 |
Target: 5'- gUGCCUGUcUCAGCCggggucggugugGCGgCGGCUUg -3' miRNA: 3'- -AUGGGCGuAGUCGGa-----------CGCgGCUGAAg -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 13385 | 0.66 | 0.475194 |
Target: 5'- aGCCCGCcUCAGUgaauaugGCGUCGACa-- -3' miRNA: 3'- aUGGGCGuAGUCGga-----CGCGGCUGaag -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 26186 | 0.67 | 0.465173 |
Target: 5'- gAUCCGCAaCGGCC-GCcCCGACcUCg -3' miRNA: 3'- aUGGGCGUaGUCGGaCGcGGCUGaAG- -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 27570 | 0.67 | 0.445468 |
Target: 5'- gUACCCGCAUCGGgC-GgGUCGAUUg- -3' miRNA: 3'- -AUGGGCGUAGUCgGaCgCGGCUGAag -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 26610 | 0.67 | 0.433872 |
Target: 5'- cACCCGCcgaucgaaccgCAGCCcGgGCCGACg-- -3' miRNA: 3'- aUGGGCGua---------GUCGGaCgCGGCUGaag -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 21885 | 0.68 | 0.407512 |
Target: 5'- cACCCGCAaguGCgUcGCGCCGACa-- -3' miRNA: 3'- aUGGGCGUaguCGgA-CGCGGCUGaag -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 9292 | 0.69 | 0.363022 |
Target: 5'- gGCgCCGCcgaaugugAUCGGCCUGUGCgCGuuuCUUCu -3' miRNA: 3'- aUG-GGCG--------UAGUCGGACGCG-GCu--GAAG- -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 10680 | 0.7 | 0.30661 |
Target: 5'- cGCCCaaaGCAUCGGCgacCUGCGCggCGGCUUUc -3' miRNA: 3'- aUGGG---CGUAGUCG---GACGCG--GCUGAAG- -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 12090 | 0.72 | 0.231943 |
Target: 5'- gUACCCGCAaCAcugguguuGCCgGCGCCGGCg-- -3' miRNA: 3'- -AUGGGCGUaGU--------CGGaCGCGGCUGaag -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 2817 | 0.72 | 0.203388 |
Target: 5'- gUugCCGUAcaAGCCUGCGCCGAagcgUCc -3' miRNA: 3'- -AugGGCGUagUCGGACGCGGCUga--AG- -5' |
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19561 | 5' | -58.4 | NC_004686.1 | + | 3494 | 1.08 | 0.000451 |
Target: 5'- aUACCCGCAUCAGCCUGCGCCGACUUCg -3' miRNA: 3'- -AUGGGCGUAGUCGGACGCGGCUGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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