Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19562 | 3' | -53.2 | NC_004686.1 | + | 11156 | 0.66 | 0.810281 |
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Target: 5'- cGUUgCGUGCGgcGAuCCGCGccgGUGCGAu -3' miRNA: 3'- cUAAgGCACGCa-CU-GGCGU---UACGCUu -5' |
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| 19562 | 3' | -53.2 | NC_004686.1 | + | 53394 | 0.67 | 0.748995 |
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Target: 5'- ---cCCGUucGCGUGGCCGCAu--CGAAu -3' miRNA: 3'- cuaaGGCA--CGCACUGGCGUuacGCUU- -5' |
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| 19562 | 3' | -53.2 | NC_004686.1 | + | 34145 | 0.67 | 0.737191 |
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Target: 5'- cGUUCCGggGUGUGAuauCCGCAGguggucaacuaucUGCGGAa -3' miRNA: 3'- cUAAGGCa-CGCACU---GGCGUU-------------ACGCUU- -5' |
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| 19562 | 3' | -53.2 | NC_004686.1 | + | 27478 | 0.7 | 0.581765 |
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Target: 5'- --aUCUGUGCGUGGgcaaucgacCCGCccGAUGCGGGu -3' miRNA: 3'- cuaAGGCACGCACU---------GGCG--UUACGCUU- -5' |
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| 19562 | 3' | -53.2 | NC_004686.1 | + | 33678 | 0.7 | 0.570583 |
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Target: 5'- --gUCCGggaaGCGUGcCCGCAaggGUGCGGg -3' miRNA: 3'- cuaAGGCa---CGCACuGGCGU---UACGCUu -5' |
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| 19562 | 3' | -53.2 | NC_004686.1 | + | 6031 | 0.7 | 0.548387 |
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Target: 5'- ---gCCGcgaaUGCGaUGGCCGCAGUGUGGGu -3' miRNA: 3'- cuaaGGC----ACGC-ACUGGCGUUACGCUU- -5' |
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| 19562 | 3' | -53.2 | NC_004686.1 | + | 48978 | 0.73 | 0.417069 |
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Target: 5'- aGAUUCCGUGCGccGuCCGCcguggcguagcccugAGUGCGAAc -3' miRNA: 3'- -CUAAGGCACGCa-CuGGCG---------------UUACGCUU- -5' |
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| 19562 | 3' | -53.2 | NC_004686.1 | + | 4688 | 1.06 | 0.002107 |
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Target: 5'- aGAUUCCGUGCGUGACCGCAAUGCGAAg -3' miRNA: 3'- -CUAAGGCACGCACUGGCGUUACGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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