miRNA: 3'- gaAGGCGAaa--CCG-CCAcAGGGCGAGu -5'
miRNA: 3'- gaAGGCGaaa-------CCGCCACAG---GGCgAGU- -5'
miRNA: 3'- gaAGGCgaAACCGCCA---CAGgGCGagu -5'
miRNA: 3'- -GAAGGCGAAac-CGCCAC-AGGGCGaGU- -5'
miRNA: 3'- -GAAGGCGAAACCGCCACAGGGCGAGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19562 | 5' | -56.7 | NC_004686.1 | + | 42466 | 0.66 | 0.62978 |
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Target: 5'- -gUCCGCUgcgaGGUaGGUuUCCCGUUCu -3' miRNA: 3'- gaAGGCGAaa--CCG-CCAcAGGGCGAGu -5' |
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| 19562 | 5' | -56.7 | NC_004686.1 | + | 48145 | 0.67 | 0.594864 |
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Target: 5'- -aUCCGCguguccuggaGGCGGUGUCgugCCGgUCGu -3' miRNA: 3'- gaAGGCGaaa-------CCGCCACAG---GGCgAGU- -5' |
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| 19562 | 5' | -56.7 | NC_004686.1 | + | 18919 | 0.67 | 0.586173 |
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Target: 5'- -gUCCGgaUUGGCGGUcucGUCgCGCa-- -3' miRNA: 3'- gaAGGCgaAACCGCCA---CAGgGCGagu -5' |
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| 19562 | 5' | -56.7 | NC_004686.1 | + | 33672 | 0.76 | 0.171544 |
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Target: 5'- cCUUCCGCUUcauGCGGUGcUCCCGC-CGg -3' miRNA: 3'- -GAAGGCGAAac-CGCCAC-AGGGCGaGU- -5' |
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| 19562 | 5' | -56.7 | NC_004686.1 | + | 4653 | 1.08 | 0.000771 |
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Target: 5'- gCUUCCGCUUUGGCGGUGUCCCGCUCAg -3' miRNA: 3'- -GAAGGCGAAACCGCCACAGGGCGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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