Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19563 | 3' | -52.3 | NC_004686.1 | + | 13038 | 0.66 | 0.854334 |
Target: 5'- -cGCgGCGCUGC-CGAGCAcccUGcUUCCAc -3' miRNA: 3'- cuUGgCGUGGCGuGCUUGU---AC-AAGGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 31270 | 0.66 | 0.854334 |
Target: 5'- cGGCCGCACgaaaugGCGCGGACcaGUG-UCCGg -3' miRNA: 3'- cUUGGCGUGg-----CGUGCUUG--UACaAGGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 10193 | 0.66 | 0.84562 |
Target: 5'- uGAACCGCGCCau-CGAACGacUGgcCCAu -3' miRNA: 3'- -CUUGGCGUGGcguGCUUGU--ACaaGGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 51344 | 0.66 | 0.84562 |
Target: 5'- --cCCGCGCCGCAguGACAU--UCCGg -3' miRNA: 3'- cuuGGCGUGGCGUgcUUGUAcaAGGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 5335 | 0.66 | 0.844736 |
Target: 5'- aGAGCCGCACCGgugaGCGggUugaugccGUGgaugCCGa -3' miRNA: 3'- -CUUGGCGUGGCg---UGCuuG-------UACaa--GGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 5619 | 0.66 | 0.836672 |
Target: 5'- --uCCGgGCCGcCACGGACGgugUCCGa -3' miRNA: 3'- cuuGGCgUGGC-GUGCUUGUacaAGGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 5583 | 0.67 | 0.827498 |
Target: 5'- cGGugUGUACCGCAccaccaaccCGAACGUGaaggCCAu -3' miRNA: 3'- -CUugGCGUGGCGU---------GCUUGUACaa--GGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 39801 | 0.67 | 0.827498 |
Target: 5'- cGGGCCGC-CUGgGCcaGGACAUGUUCa- -3' miRNA: 3'- -CUUGGCGuGGCgUG--CUUGUACAAGgu -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 39305 | 0.68 | 0.778616 |
Target: 5'- -cACCGCACCGUGC-AGCGUGc-CCGc -3' miRNA: 3'- cuUGGCGUGGCGUGcUUGUACaaGGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 28185 | 0.68 | 0.778616 |
Target: 5'- aGAGCaGCACCGCcaGCGAugcucaACGUGgcuUUCCAg -3' miRNA: 3'- -CUUGgCGUGGCG--UGCU------UGUAC---AAGGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 1908 | 0.69 | 0.714857 |
Target: 5'- -cGCCGCACCGguCGAAuCAUGcuugauaggugUCCGu -3' miRNA: 3'- cuUGGCGUGGCguGCUU-GUACa----------AGGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 4707 | 0.69 | 0.692789 |
Target: 5'- aGACCGCGCCGCG-GAGCAggcgaaugCCGa -3' miRNA: 3'- cUUGGCGUGGCGUgCUUGUacaa----GGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 48347 | 0.69 | 0.681657 |
Target: 5'- gGGAUCGaACCGgGCGAACAUGUcuauagCCAu -3' miRNA: 3'- -CUUGGCgUGGCgUGCUUGUACAa-----GGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 45603 | 0.69 | 0.670476 |
Target: 5'- aGGCUGCgcaACCGCAUGuACGUGUUCg- -3' miRNA: 3'- cUUGGCG---UGGCGUGCuUGUACAAGgu -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 14550 | 0.69 | 0.670476 |
Target: 5'- cGGgUGCACUGCACGGACA-GUUCg- -3' miRNA: 3'- cUUgGCGUGGCGUGCUUGUaCAAGgu -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 39802 | 0.7 | 0.648011 |
Target: 5'- cGAACCGCGuuGCGCc-GCAaGUUCCc -3' miRNA: 3'- -CUUGGCGUggCGUGcuUGUaCAAGGu -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 42390 | 0.7 | 0.625485 |
Target: 5'- gGAACCGCAUCaGCACccGCAUcaGUUCCu -3' miRNA: 3'- -CUUGGCGUGG-CGUGcuUGUA--CAAGGu -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 15604 | 0.72 | 0.536395 |
Target: 5'- uGAGCCGCACUGgcugaccucacCugGGACGUggGUUCCGg -3' miRNA: 3'- -CUUGGCGUGGC-----------GugCUUGUA--CAAGGU- -5' |
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19563 | 3' | -52.3 | NC_004686.1 | + | 6465 | 1.09 | 0.002082 |
Target: 5'- aGAACCGCACCGCACGAACAUGUUCCAc -3' miRNA: 3'- -CUUGGCGUGGCGUGCUUGUACAAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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