Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19563 | 5' | -53.1 | NC_004686.1 | + | 28993 | 0.66 | 0.813632 |
Target: 5'- -uCAGGGguGuguGUCGGCGuggGGUGGUGc- -3' miRNA: 3'- cuGUCUCguC---UAGCCGC---UCACCAUua -5' |
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19563 | 5' | -53.1 | NC_004686.1 | + | 26440 | 0.66 | 0.79394 |
Target: 5'- ---uGGGCAGGUCggGGCGGGgGGUGGg -3' miRNA: 3'- cuguCUCGUCUAG--CCGCUCaCCAUUa -5' |
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19563 | 5' | -53.1 | NC_004686.1 | + | 35509 | 0.66 | 0.77353 |
Target: 5'- uGGCAuGAGUcGcgCGGUGAGUGGgugAAUg -3' miRNA: 3'- -CUGU-CUCGuCuaGCCGCUCACCa--UUA- -5' |
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19563 | 5' | -53.1 | NC_004686.1 | + | 23941 | 0.67 | 0.763087 |
Target: 5'- -cCAGAGUgaAGAUCGGCGAa-GGUGu- -3' miRNA: 3'- cuGUCUCG--UCUAGCCGCUcaCCAUua -5' |
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19563 | 5' | -53.1 | NC_004686.1 | + | 38030 | 0.67 | 0.762035 |
Target: 5'- uGACGG-GCAGGUCGGCccaguugGuuUGGUGGUu -3' miRNA: 3'- -CUGUCuCGUCUAGCCG-------CucACCAUUA- -5' |
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19563 | 5' | -53.1 | NC_004686.1 | + | 53756 | 0.67 | 0.752501 |
Target: 5'- aGACGGAGCAGAUCaGGauCGAcagaucUGGUGGUu -3' miRNA: 3'- -CUGUCUCGUCUAG-CC--GCUc-----ACCAUUA- -5' |
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19563 | 5' | -53.1 | NC_004686.1 | + | 31137 | 0.67 | 0.720012 |
Target: 5'- uGACGGAGaAGAuUCGGCGugcuGUGGUc-- -3' miRNA: 3'- -CUGUCUCgUCU-AGCCGCu---CACCAuua -5' |
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19563 | 5' | -53.1 | NC_004686.1 | + | 22630 | 0.68 | 0.664178 |
Target: 5'- gGugAGGGCGGcugCGGCGGGUuGUAGg -3' miRNA: 3'- -CugUCUCGUCua-GCCGCUCAcCAUUa -5' |
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19563 | 5' | -53.1 | NC_004686.1 | + | 27498 | 0.69 | 0.6302 |
Target: 5'- aGACcccGGCGGAagUCGGCGAGccGGUAGUg -3' miRNA: 3'- -CUGuc-UCGUCU--AGCCGCUCa-CCAUUA- -5' |
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19563 | 5' | -53.1 | NC_004686.1 | + | 19278 | 0.69 | 0.630199 |
Target: 5'- uGugAGGGCGGu---GCGGGUGGUGAUu -3' miRNA: 3'- -CugUCUCGUCuagcCGCUCACCAUUA- -5' |
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19563 | 5' | -53.1 | NC_004686.1 | + | 6431 | 1.05 | 0.002512 |
Target: 5'- aGACAGAGCAGAUCGGCGAGUGGUAAUu -3' miRNA: 3'- -CUGUCUCGUCUAGCCGCUCACCAUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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