Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19564 | 5' | -54.2 | NC_004686.1 | + | 15851 | 0.66 | 0.807648 |
Target: 5'- uGUCG-UCGuAGCgUGUGccGUAGUCGCCg -3' miRNA: 3'- -CGGCuAGCcUCGaACAU--CAUCGGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 12196 | 0.66 | 0.805743 |
Target: 5'- cGCCGuugCGGcAGCcgaagccgcGGUAGCCGCa -3' miRNA: 3'- -CGGCua-GCC-UCGaaca-----UCAUCGGCGg -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 50724 | 0.66 | 0.768277 |
Target: 5'- uGgCGAUCaGGGC--GUGGaccugAGCCGCCu -3' miRNA: 3'- -CgGCUAGcCUCGaaCAUCa----UCGGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 54320 | 0.66 | 0.766243 |
Target: 5'- uGCCGGUaggcguUGGGGCUguguuccgcggcGUAcUGGCUGCCa -3' miRNA: 3'- -CGGCUA------GCCUCGAa-----------CAUcAUCGGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 33545 | 0.66 | 0.758055 |
Target: 5'- aCCGaAUCGGAGCUgauggcacugGUGG-GGCUGUUa -3' miRNA: 3'- cGGC-UAGCCUCGAa---------CAUCaUCGGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 24558 | 0.66 | 0.754962 |
Target: 5'- aCCGGUCaccaGGuGUcgGUGGUcucgacaugguggcGGCCGCCg -3' miRNA: 3'- cGGCUAG----CCuCGaaCAUCA--------------UCGGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 9959 | 0.67 | 0.730901 |
Target: 5'- cGCCGAUCGuGGGUUgccgaucucacGUGGCUGCa -3' miRNA: 3'- -CGGCUAGC-CUCGAacau-------CAUCGGCGg -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 36748 | 0.68 | 0.672592 |
Target: 5'- cGCCGAUCGGcacGGUc-GUGGUGacgacCCGCCc -3' miRNA: 3'- -CGGCUAGCC---UCGaaCAUCAUc----GGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 53310 | 0.68 | 0.672592 |
Target: 5'- uGCUGGugUCGGuGCUgcUGUgcGGUuuuGGCUGCCg -3' miRNA: 3'- -CGGCU--AGCCuCGA--ACA--UCA---UCGGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 17874 | 0.68 | 0.66162 |
Target: 5'- cGCCGAgugCGGcGgaUGUGG-AGUgGCCg -3' miRNA: 3'- -CGGCUa--GCCuCgaACAUCaUCGgCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 11915 | 0.69 | 0.628579 |
Target: 5'- gGCCGGUCGGuGCcgGUGGgcaacCCGUCc -3' miRNA: 3'- -CGGCUAGCCuCGaaCAUCauc--GGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 20986 | 0.69 | 0.617558 |
Target: 5'- cGCCGAUCacugguauugGGGGCgguacggGUAGUuucGCUGCUg -3' miRNA: 3'- -CGGCUAG----------CCUCGaa-----CAUCAu--CGGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 14053 | 0.69 | 0.60655 |
Target: 5'- cGCUGGUCGGgccGGCUugauaagugUGUGGUGGaguaCCGCg -3' miRNA: 3'- -CGGCUAGCC---UCGA---------ACAUCAUC----GGCGg -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 53409 | 0.72 | 0.458371 |
Target: 5'- uGUCGA-CGGAGCUggcccGUucgcGUGGCCGCa -3' miRNA: 3'- -CGGCUaGCCUCGAa----CAu---CAUCGGCGg -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 11381 | 0.72 | 0.438701 |
Target: 5'- aGCCGAUcgaccCGGAcgauuaccuGUUUccggaGUGGUGGCCGCCc -3' miRNA: 3'- -CGGCUA-----GCCU---------CGAA-----CAUCAUCGGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 3637 | 0.72 | 0.419524 |
Target: 5'- gGUCGAUCaGAGCUUGcAGcguGCCGCg -3' miRNA: 3'- -CGGCUAGcCUCGAACaUCau-CGGCGg -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 6993 | 0.77 | 0.238918 |
Target: 5'- uCCGAUCGGcuGGUggUGUGGUggcguucgaggaAGCCGCCg -3' miRNA: 3'- cGGCUAGCC--UCGa-ACAUCA------------UCGGCGG- -5' |
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19564 | 5' | -54.2 | NC_004686.1 | + | 7059 | 1.15 | 0.000536 |
Target: 5'- aGCCGAUCGGAGCUUGUAGUAGCCGCCg -3' miRNA: 3'- -CGGCUAGCCUCGAACAUCAUCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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