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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19566 | 5' | -59.4 | NC_004686.1 | + | 8507 | 0.91 | 0.009922 |
Target: 5'- cCGACAGUGGGGCUUgC-CCUUCGGGGUg -3' miRNA: 3'- -GCUGUCACCCCGAA-GcGGAAGCCCCA- -5' |
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19566 | 5' | -59.4 | NC_004686.1 | + | 12461 | 0.69 | 0.35318 |
Target: 5'- gGGCAaUGGGGCacCGCCgggacCGGGGg -3' miRNA: 3'- gCUGUcACCCCGaaGCGGaa---GCCCCa -5' |
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19566 | 5' | -59.4 | NC_004686.1 | + | 18401 | 0.67 | 0.441547 |
Target: 5'- uCGAUAa--GGGUUugUUGCCUUCGGGGg -3' miRNA: 3'- -GCUGUcacCCCGA--AGCGGAAGCCCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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