Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19567 | 5' | -53.8 | NC_004686.1 | + | 36865 | 0.65 | 0.835824 |
Target: 5'- aCUCgCugaguUCGgCGAGGGCUGCUCAuaaggugauggcgUCGGa -3' miRNA: 3'- -GAG-Gu----GGUgGCUCUUGACGAGU-------------AGCC- -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 49306 | 0.66 | 0.827744 |
Target: 5'- uUCCugCGCCcGGAGa-GCUCAacuUCGGc -3' miRNA: 3'- gAGGugGUGGcUCUUgaCGAGU---AGCC- -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 5259 | 0.66 | 0.81857 |
Target: 5'- -cCCGCuCACCGGu-GCgGCUCuGUCGGg -3' miRNA: 3'- gaGGUG-GUGGCUcuUGaCGAG-UAGCC- -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 22204 | 0.66 | 0.81857 |
Target: 5'- ---uGCCGCCGAGGccgcgcgcgccACUGCUCGaCGa -3' miRNA: 3'- gaggUGGUGGCUCU-----------UGACGAGUaGCc -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 23585 | 0.66 | 0.8092 |
Target: 5'- gUCCACCACCu-GGGCUGgugUCAcCGGu -3' miRNA: 3'- gAGGUGGUGGcuCUUGACg--AGUaGCC- -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 47995 | 0.66 | 0.799643 |
Target: 5'- uUCCACuUGCCGGucACUGUUCGUCu- -3' miRNA: 3'- gAGGUG-GUGGCUcuUGACGAGUAGcc -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 40171 | 0.66 | 0.798678 |
Target: 5'- ---gGCCGCCGAagaugccGAACUGUUCGUUGu -3' miRNA: 3'- gaggUGGUGGCU-------CUUGACGAGUAGCc -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 14256 | 0.67 | 0.780015 |
Target: 5'- cCUCCAgCugauCCGGGAACacaacccGCUCAUCa- -3' miRNA: 3'- -GAGGUgGu---GGCUCUUGa------CGAGUAGcc -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 35188 | 0.67 | 0.780015 |
Target: 5'- aUCCACCGCUu-GAACgGCUuCAcccUCGGa -3' miRNA: 3'- gAGGUGGUGGcuCUUGaCGA-GU---AGCC- -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 27071 | 0.67 | 0.779017 |
Target: 5'- uCUUCACCGuuGAGGGCcacaugucgaacaUGUUCuUCGGu -3' miRNA: 3'- -GAGGUGGUggCUCUUG-------------ACGAGuAGCC- -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 3428 | 0.67 | 0.74945 |
Target: 5'- uUCCACuCAUCGGGGAU--CUCGUCGa -3' miRNA: 3'- gAGGUG-GUGGCUCUUGacGAGUAGCc -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 26994 | 0.67 | 0.74945 |
Target: 5'- cCUCCGCCgucaacGCCGAGGugaacaggucACUG-UCGUCGa -3' miRNA: 3'- -GAGGUGG------UGGCUCU----------UGACgAGUAGCc -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 49025 | 0.68 | 0.727396 |
Target: 5'- --gCACCACUGAGGggccgugGCUGUUCAUuccCGGc -3' miRNA: 3'- gagGUGGUGGCUCU-------UGACGAGUA---GCC- -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 49730 | 0.69 | 0.641532 |
Target: 5'- -cUCACCGCUGAGAGCcgGC-CAcgCGGg -3' miRNA: 3'- gaGGUGGUGGCUCUUGa-CGaGUa-GCC- -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 19860 | 0.69 | 0.630516 |
Target: 5'- cCUCCACCACCGcAGAAg-GUUCGgacUCGu -3' miRNA: 3'- -GAGGUGGUGGC-UCUUgaCGAGU---AGCc -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 1152 | 0.71 | 0.542143 |
Target: 5'- -gCCACCACCGGGuGCUGggCAcgcaucuUCGGc -3' miRNA: 3'- gaGGUGGUGGCUCuUGACgaGU-------AGCC- -5' |
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19567 | 5' | -53.8 | NC_004686.1 | + | 8840 | 1.1 | 0.00137 |
Target: 5'- uCUCCACCACCGAGAACUGCUCAUCGGu -3' miRNA: 3'- -GAGGUGGUGGCUCUUGACGAGUAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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