miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
19568 3' -64.2 NC_004686.1 + 8921 0.68 0.231629
Target:  5'- cCGCGGAUGGaCCauccgagacaucggCCCCCGaa-UCUCCu -3'
miRNA:   3'- -GCGCCUACC-GG--------------GGGGGCacgAGAGGc -5'
19568 3' -64.2 NC_004686.1 + 37235 0.69 0.211426
Target:  5'- gCGCGGGcaUGGUUCCCCC--GCUCcCCGc -3'
miRNA:   3'- -GCGCCU--ACCGGGGGGGcaCGAGaGGC- -5'
19568 3' -64.2 NC_004686.1 + 8963 1.07 0.000221
Target:  5'- gCGCGGAUGGCCCCCCCGUGCUCUCCGu -3'
miRNA:   3'- -GCGCCUACCGGGGGGGCACGAGAGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.