Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19568 | 5' | -55.3 | NC_004686.1 | + | 56176 | 0.66 | 0.719418 |
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Target: 5'- -gUGGGGGGGuccCCGACcccgacCCCCCAGa -3' miRNA: 3'- caGCCUCUCC---GGUUGuuaa--GGGGGUC- -5' |
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| 19568 | 5' | -55.3 | NC_004686.1 | + | 9056 | 0.66 | 0.718346 |
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Target: 5'- uUCGGuGGGaGCCAgGCGAUcaggucgcggacgUCaCCCCAGg -3' miRNA: 3'- cAGCCuCUC-CGGU-UGUUA-------------AG-GGGGUC- -5' |
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| 19568 | 5' | -55.3 | NC_004686.1 | + | 40789 | 0.67 | 0.620805 |
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Target: 5'- uGUCuGAGGGGCCGccuuuGCAGUUCCUggaCCGc -3' miRNA: 3'- -CAGcCUCUCCGGU-----UGUUAAGGG---GGUc -5' |
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| 19568 | 5' | -55.3 | NC_004686.1 | + | 43492 | 0.68 | 0.598736 |
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Target: 5'- aUCGGGGGuGCCAACAugagaUCCCCAa -3' miRNA: 3'- cAGCCUCUcCGGUUGUuaa--GGGGGUc -5' |
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| 19568 | 5' | -55.3 | NC_004686.1 | + | 11809 | 0.68 | 0.58774 |
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Target: 5'- -gCGaAGGGcGCCAACGAcUUCUCCCAGc -3' miRNA: 3'- caGCcUCUC-CGGUUGUU-AAGGGGGUC- -5' |
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| 19568 | 5' | -55.3 | NC_004686.1 | + | 8929 | 1.07 | 0.001227 |
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Target: 5'- gGUCGGAGAGGCCAACAAUUCCCCCAGc -3' miRNA: 3'- -CAGCCUCUCCGGUUGUUAAGGGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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