Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19569 | 3' | -50.5 | NC_004686.1 | + | 37156 | 0.66 | 0.939064 |
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Target: 5'- gUCCacgaGCCUCGUUcuucUGCCGAacGUCGGg -3' miRNA: 3'- gAGG----CGGAGUAAcuu-ACGGCU--UAGCU- -5' |
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| 19569 | 3' | -50.5 | NC_004686.1 | + | 5311 | 0.66 | 0.939064 |
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Target: 5'- --aUGCCg---UGGAUGCCGAucagGUCGAg -3' miRNA: 3'- gagGCGGaguaACUUACGGCU----UAGCU- -5' |
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| 19569 | 3' | -50.5 | NC_004686.1 | + | 55346 | 0.66 | 0.93365 |
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Target: 5'- -cCCGCUUCGUUGAcgGCCc---CGGc -3' miRNA: 3'- gaGGCGGAGUAACUuaCGGcuuaGCU- -5' |
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| 19569 | 3' | -50.5 | NC_004686.1 | + | 5258 | 0.66 | 0.927942 |
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Target: 5'- gUCCGCCauggUCugcaGGAcGCCGAGUUGAc -3' miRNA: 3'- gAGGCGG----AGuaa-CUUaCGGCUUAGCU- -5' |
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| 19569 | 3' | -50.5 | NC_004686.1 | + | 4355 | 0.66 | 0.921939 |
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Target: 5'- gUCCGUC-CGUUGAccGCCGGAcgUGGg -3' miRNA: 3'- gAGGCGGaGUAACUuaCGGCUUa-GCU- -5' |
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| 19569 | 3' | -50.5 | NC_004686.1 | + | 7517 | 0.66 | 0.921323 |
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Target: 5'- aCUUCGCCgCAccaugcuucgaugUUG-AUGCUGGAUCGGa -3' miRNA: 3'- -GAGGCGGaGU-------------AACuUACGGCUUAGCU- -5' |
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| 19569 | 3' | -50.5 | NC_004686.1 | + | 28918 | 0.69 | 0.799003 |
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Target: 5'- uUCgGUCaCGUUGGcgGUCGAAUCGAu -3' miRNA: 3'- gAGgCGGaGUAACUuaCGGCUUAGCU- -5' |
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| 19569 | 3' | -50.5 | NC_004686.1 | + | 52336 | 0.72 | 0.646934 |
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Target: 5'- -gCUGCCUCAcUGGAUcGCCGGA-CGAg -3' miRNA: 3'- gaGGCGGAGUaACUUA-CGGCUUaGCU- -5' |
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| 19569 | 3' | -50.5 | NC_004686.1 | + | 17200 | 0.74 | 0.549857 |
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Target: 5'- aCUCgGCaagggacccauaGUUGAAUGCCGAAUCGGg -3' miRNA: 3'- -GAGgCGgag---------UAACUUACGGCUUAGCU- -5' |
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| 19569 | 3' | -50.5 | NC_004686.1 | + | 9418 | 1.1 | 0.002676 |
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Target: 5'- aCUCCGCCUCAUUGAAUGCCGAAUCGAu -3' miRNA: 3'- -GAGGCGGAGUAACUUACGGCUUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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