Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19569 | 5' | -51.5 | NC_004686.1 | + | 38867 | 0.66 | 0.875617 |
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Target: 5'- --cCGUg-CAGCCGCAGUGCcugcagugGGCGUu -3' miRNA: 3'- aaaGCAgaGUCGGUGUUACGa-------CUGCA- -5' |
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| 19569 | 5' | -51.5 | NC_004686.1 | + | 49769 | 0.66 | 0.875617 |
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Target: 5'- -gUCGauuucCUCGGCCACAugcGCUG-CGUg -3' miRNA: 3'- aaAGCa----GAGUCGGUGUua-CGACuGCA- -5' |
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| 19569 | 5' | -51.5 | NC_004686.1 | + | 31647 | 0.66 | 0.867376 |
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Target: 5'- --cCGUaaUUCGGCgGCGAUGCaGGCGUu -3' miRNA: 3'- aaaGCA--GAGUCGgUGUUACGaCUGCA- -5' |
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| 19569 | 5' | -51.5 | NC_004686.1 | + | 30043 | 0.66 | 0.858867 |
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Target: 5'- --cUGUCUCAGCCGggguCGGUGUggcGGCGg -3' miRNA: 3'- aaaGCAGAGUCGGU----GUUACGa--CUGCa -5' |
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| 19569 | 5' | -51.5 | NC_004686.1 | + | 26485 | 0.66 | 0.841084 |
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Target: 5'- --gCGUCaUCGGCCACAaggagugggccgGUGCUG-CGc -3' miRNA: 3'- aaaGCAG-AGUCGGUGU------------UACGACuGCa -5' |
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| 19569 | 5' | -51.5 | NC_004686.1 | + | 7117 | 0.69 | 0.683632 |
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Target: 5'- --aCGg--CGGCCACAAggacGCUGGCGUa -3' miRNA: 3'- aaaGCagaGUCGGUGUUa---CGACUGCA- -5' |
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| 19569 | 5' | -51.5 | NC_004686.1 | + | 9376 | 0.96 | 0.015278 |
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Target: 5'- gUUUCG-CUCAGCCACAAUGCUGACGUa -3' miRNA: 3'- -AAAGCaGAGUCGGUGUUACGACUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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