Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19570 | 3' | -56 | NC_004686.1 | + | 40442 | 0.66 | 0.718441 |
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Target: 5'- aCCUC-CCGaGUucaCACCaaGUACUGCUc- -3' miRNA: 3'- -GGAGuGGC-CA---GUGGggCAUGACGAag -5' |
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| 19570 | 3' | -56 | NC_004686.1 | + | 2634 | 0.66 | 0.715272 |
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Target: 5'- uCCUCACCaacaguguuaaucaGGaaCGCCggaCCGUGCUGCaUCg -3' miRNA: 3'- -GGAGUGG--------------CCa-GUGG---GGCAUGACGaAG- -5' |
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| 19570 | 3' | -56 | NC_004686.1 | + | 2871 | 0.66 | 0.70785 |
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Target: 5'- aCCUgCGCCGG-CACaCCGUAaacaUGCUcaUCa -3' miRNA: 3'- -GGA-GUGGCCaGUGgGGCAUg---ACGA--AG- -5' |
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| 19570 | 3' | -56 | NC_004686.1 | + | 21717 | 0.66 | 0.704658 |
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Target: 5'- -gUCACUGGaggaACCCCGUcggucgccgugaagGCUGCgUUCg -3' miRNA: 3'- ggAGUGGCCag--UGGGGCA--------------UGACG-AAG- -5' |
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| 19570 | 3' | -56 | NC_004686.1 | + | 13387 | 0.66 | 0.697187 |
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Target: 5'- -gUCACCGGcggaACCCCGacGCUGCc-- -3' miRNA: 3'- ggAGUGGCCag--UGGGGCa-UGACGaag -5' |
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| 19570 | 3' | -56 | NC_004686.1 | + | 30766 | 0.67 | 0.632284 |
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Target: 5'- uCCUC-CUGGUCggcuguaugACCCUGUGgaGUUUCc -3' miRNA: 3'- -GGAGuGGCCAG---------UGGGGCAUgaCGAAG- -5' |
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| 19570 | 3' | -56 | NC_004686.1 | + | 49093 | 0.67 | 0.610542 |
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Target: 5'- cCCUCAgUGGU-GCCUuccaGUGCUGCUUUc -3' miRNA: 3'- -GGAGUgGCCAgUGGGg---CAUGACGAAG- -5' |
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| 19570 | 3' | -56 | NC_004686.1 | + | 42123 | 0.69 | 0.504222 |
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Target: 5'- --aCACCGGUgAaaaaCCCGUugUGCUg- -3' miRNA: 3'- ggaGUGGCCAgUg---GGGCAugACGAag -5' |
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| 19570 | 3' | -56 | NC_004686.1 | + | 3324 | 0.69 | 0.493983 |
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Target: 5'- gCCUCAgCGaUCAgCUCGUACUGCg-- -3' miRNA: 3'- -GGAGUgGCcAGUgGGGCAUGACGaag -5' |
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| 19570 | 3' | -56 | NC_004686.1 | + | 10076 | 1.11 | 0.000694 |
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Target: 5'- gCCUCACCGGUCACCCCGUACUGCUUCu -3' miRNA: 3'- -GGAGUGGCCAGUGGGGCAUGACGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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