miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
19571 3' -56.3 NC_004686.1 + 15782 0.67 0.58578
Target:  5'- gCGGAacacuUCGGCCUGCUGCugcgGCgUCAGc -3'
miRNA:   3'- -GCCUau---AGCCGGACGGUG----CGgAGUUu -5'
19571 3' -56.3 NC_004686.1 + 39441 0.67 0.574821
Target:  5'- uGGGUGUUGGgCagGCCACGaUCUCGGc -3'
miRNA:   3'- gCCUAUAGCCgGa-CGGUGC-GGAGUUu -5'
19571 3' -56.3 NC_004686.1 + 13776 0.69 0.459008
Target:  5'- aGGcgAUCuGGCgUGCCACGCUcgcgCAGAa -3'
miRNA:   3'- gCCuaUAG-CCGgACGGUGCGGa---GUUU- -5'
19571 3' -56.3 NC_004686.1 + 29121 0.69 0.42956
Target:  5'- gGGAUcggcGUCGGCggGCCGCGCUUgCGGAu -3'
miRNA:   3'- gCCUA----UAGCCGgaCGGUGCGGA-GUUU- -5'
19571 3' -56.3 NC_004686.1 + 975 0.69 0.42956
Target:  5'- ------cCGGCCUGCCGCGCaaugCUCAGGg -3'
miRNA:   3'- gccuauaGCCGGACGGUGCG----GAGUUU- -5'
19571 3' -56.3 NC_004686.1 + 10493 1.06 0.001179
Target:  5'- gCGGAUAUCGGCCUGCCACGCCUCAAAc -3'
miRNA:   3'- -GCCUAUAGCCGGACGGUGCGGAGUUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.