Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19571 | 5' | -50.1 | NC_004686.1 | + | 11676 | 0.66 | 0.936485 |
Target: 5'- gGGCUUCAAGCccggaGCAUugaaUGUCGGaACCAa -3' miRNA: 3'- -CCGAGGUUCG-----UGUA----ACGGUUaUGGUg -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 22490 | 0.66 | 0.93426 |
Target: 5'- aGGCUgguacagCCAgucgaugcgguuguAGC-CAUcggUGCCGGUGCCGCc -3' miRNA: 3'- -CCGA-------GGU--------------UCGuGUA---ACGGUUAUGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 19590 | 0.66 | 0.930831 |
Target: 5'- cGGCUCCGggguaGGCGCAU--CC---ACCGCa -3' miRNA: 3'- -CCGAGGU-----UCGUGUAacGGuuaUGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 33500 | 0.66 | 0.924872 |
Target: 5'- uGGCaggCCGAGCACAUggUGCC----CCAg -3' miRNA: 3'- -CCGa--GGUUCGUGUA--ACGGuuauGGUg -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 26307 | 0.66 | 0.924872 |
Target: 5'- uGGCUgCCGAcaaACAacGCCAAUGCgCACa -3' miRNA: 3'- -CCGA-GGUUcg-UGUaaCGGUUAUG-GUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 6496 | 0.66 | 0.923643 |
Target: 5'- uGGCUCCugcucguagaucGGCugAcccGCCAGaACCGCa -3' miRNA: 3'- -CCGAGGu-----------UCGugUaa-CGGUUaUGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 39646 | 0.66 | 0.91204 |
Target: 5'- uGCUCCGAccGCACAgUGUCAcgggugaggGUGCgGCa -3' miRNA: 3'- cCGAGGUU--CGUGUaACGGU---------UAUGgUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 56147 | 0.66 | 0.91204 |
Target: 5'- cGGgUCCAccGGuCGCGggUGCCGA-ACCGCc -3' miRNA: 3'- -CCgAGGU--UC-GUGUa-ACGGUUaUGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 49783 | 0.67 | 0.897268 |
Target: 5'- -cCUCCGAGgGCuUUGUCGAUuuccucgGCCACa -3' miRNA: 3'- ccGAGGUUCgUGuAACGGUUA-------UGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 4213 | 0.67 | 0.882783 |
Target: 5'- cGGCUCCAccGGCAac--GCUug-ACCACc -3' miRNA: 3'- -CCGAGGU--UCGUguaaCGGuuaUGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 9594 | 0.67 | 0.882783 |
Target: 5'- cGUUCCGAcaGCGUUGCCGAUcuucuccCCACg -3' miRNA: 3'- cCGAGGUUcgUGUAACGGUUAu------GGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 43810 | 0.67 | 0.882783 |
Target: 5'- cGGC-CCAGGUugcgUGCCGcgACCAa -3' miRNA: 3'- -CCGaGGUUCGuguaACGGUuaUGGUg -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 54808 | 0.67 | 0.874746 |
Target: 5'- cGGCgcauUCCcggaAGGCGCGUUGCUcGUGauCCACa -3' miRNA: 3'- -CCG----AGG----UUCGUGUAACGGuUAU--GGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 55943 | 0.68 | 0.849005 |
Target: 5'- cGCgCCAucAGCACAUgGaCCGAUACCGu -3' miRNA: 3'- cCGaGGU--UCGUGUAaC-GGUUAUGGUg -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 23318 | 0.68 | 0.849005 |
Target: 5'- cGGUaaauUCCGAGUAgG-UGCCuguUGCCGCa -3' miRNA: 3'- -CCG----AGGUUCGUgUaACGGuu-AUGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 51185 | 0.68 | 0.827729 |
Target: 5'- aGGCugUCCAGGUACA--GCCucgcggcggacgagAAUGCCGCc -3' miRNA: 3'- -CCG--AGGUUCGUGUaaCGG--------------UUAUGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 52700 | 0.7 | 0.759438 |
Target: 5'- cGCUCCAccAGCcaggaguaGUUGCCG-UGCCGCu -3' miRNA: 3'- cCGAGGU--UCGug------UAACGGUuAUGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 21299 | 0.7 | 0.748597 |
Target: 5'- uGGC-CCAGGCACcgcugcUGaCCGAcACCACa -3' miRNA: 3'- -CCGaGGUUCGUGua----AC-GGUUaUGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 13036 | 0.7 | 0.748597 |
Target: 5'- cGGCgcugCCGAGCACccUGCUu---CCACg -3' miRNA: 3'- -CCGa---GGUUCGUGuaACGGuuauGGUG- -5' |
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19571 | 5' | -50.1 | NC_004686.1 | + | 26422 | 0.7 | 0.748597 |
Target: 5'- gGGUgggCCAGGCGCAU--UCGAUGCCGa -3' miRNA: 3'- -CCGa--GGUUCGUGUAacGGUUAUGGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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