Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19572 | 3' | -55.3 | NC_004686.1 | + | 4818 | 0.67 | 0.635159 |
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Target: 5'- uUCGGaUGUUUcuucuUGCGCUcgGCCUCGGCAu- -3' miRNA: 3'- -GGUC-GCAAG-----ACGCGA--CGGAGUCGUuc -5' |
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| 19572 | 3' | -55.3 | NC_004686.1 | + | 32419 | 0.67 | 0.612992 |
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Target: 5'- aCGGCGaaugUCUGCcgGCUGaagaUCGGCAAGu -3' miRNA: 3'- gGUCGCa---AGACG--CGACgg--AGUCGUUC- -5' |
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| 19572 | 3' | -55.3 | NC_004686.1 | + | 18686 | 0.67 | 0.610777 |
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Target: 5'- aCCAGCuugacgagGCGCUGCCccgcgagaUCAGCGAc -3' miRNA: 3'- -GGUCGcaaga---CGCGACGG--------AGUCGUUc -5' |
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| 19572 | 3' | -55.3 | NC_004686.1 | + | 38250 | 0.68 | 0.590892 |
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Target: 5'- gCgGGCGUUCaugcUGCGuCUcCCUCGGUGAGg -3' miRNA: 3'- -GgUCGCAAG----ACGC-GAcGGAGUCGUUC- -5' |
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| 19572 | 3' | -55.3 | NC_004686.1 | + | 48450 | 0.68 | 0.568933 |
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Target: 5'- aCUGGCcUUCgGCGCUGuUCUCGGCGGa -3' miRNA: 3'- -GGUCGcAAGaCGCGAC-GGAGUCGUUc -5' |
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| 19572 | 3' | -55.3 | NC_004686.1 | + | 22122 | 0.69 | 0.525702 |
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Target: 5'- gCAGUGgcgcGCGCgGCCUCGGCGGc -3' miRNA: 3'- gGUCGCaagaCGCGaCGGAGUCGUUc -5' |
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| 19572 | 3' | -55.3 | NC_004686.1 | + | 3354 | 0.69 | 0.524636 |
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Target: 5'- gCCGGCGUgaaaucaUCcaccGCGCcuucaGCCUCAGCGAu -3' miRNA: 3'- -GGUCGCA-------AGa---CGCGa----CGGAGUCGUUc -5' |
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| 19572 | 3' | -55.3 | NC_004686.1 | + | 34796 | 0.7 | 0.463411 |
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Target: 5'- gCCGGCGaacUUCUGcCGCUGCCaccgauucgcuUCGGCu-- -3' miRNA: 3'- -GGUCGC---AAGAC-GCGACGG-----------AGUCGuuc -5' |
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| 19572 | 3' | -55.3 | NC_004686.1 | + | 41024 | 0.75 | 0.247484 |
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Target: 5'- aUCAGCGgcUUGCGCUcGCUUCAGUAGu -3' miRNA: 3'- -GGUCGCaaGACGCGA-CGGAGUCGUUc -5' |
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| 19572 | 3' | -55.3 | NC_004686.1 | + | 10607 | 1.11 | 0.000607 |
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Target: 5'- cCCAGCGUUCUGCGCUGCCUCAGCAAGg -3' miRNA: 3'- -GGUCGCAAGACGCGACGGAGUCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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